Reaction mass of 2-methyldecan-1-al and undecanal

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

test start: 06 Aug. 1990; test end: 08 Aug. 1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 79/831/EEC

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Undecanal

EC Number:

203-972-6

EC Name:

Undecanal

Cas Number:

112-44-7

Molecular formula:

C11H22O

IUPAC Name:

undecanal

Details on test material:

- Name of test material (as cited in study report): undecanal
- Physical state: liquid
- Analytical purity: ca. 65%
- Impurities (identity and concentrations): decane, undecanol, 2- methyl decanal
- Lot/batch No.: product no. 072525, lot no. 5/89

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution: The stock solution (100 mg/L test item) was prepared with Cremophor RH 40. A dilution series was prepared with dilution water. 10 mL of the prepared diluted solutions were added to the test vessels.
- Controls: negative control, solvent control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Cremophor RH 40
- Concentration of vehicle in test medium (stock solution and final test solution): stock solution 100 mg/L Cremophor RH 40
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: originally supplied from 'Institut National de Recherche Chimique appliquee, France', and has been bred from 1978 in the Laboratories of BASF
- Age at study initiation (mean and range, SD): 2-26 h
- Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

test start: 2.88 mmol/L

Test temperature:

20±1 °C

pH:

test start: 7.6 to 8.1
test end: 7.5 to 8.0

Dissolved oxygen:

test start: 8.3 to 9.1 mg/L
test end: 6.1 to 9.1 mg/L

Nominal and measured concentrations:

nominal (test material): 0 (control), 0 (solvent control), 0.78, 1.56, 3.13, 6.25, 12.5, 25, 50, and 100 mg/L
nominal (active ingredient): 0 (control), 0 (solvent control), 0.51, 1.01, 2.03, 4.06, 8.12, 16.25, 32.5, and 65 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: test tubes with flat bottom
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glas, 20-mL total volume, 10-mL fill volume
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate: 1 animal/2 mL

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Alkalinity: 0.80 to 1.20 mmol/L
- Ca/mg ratio: 4:1
- Conductivity: 678 µSiemens/cm at test start
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: continuous darkness
- Light intensity: not applicable

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- pH and dissolved oxygen: 0 and 48 h
- immobile animals: 0, 3, 6, 24, and 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study
- Test concentrations: no data
- Results used to determine the conditions for the definitive study: no data

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.5 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Remarks on result:

other: calculated from purity data

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.85 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: not noted
- Mortality of control: 0%; solent control: 0%
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: yes (solvent was used to dissolve test item)

Any other information on results incl. tables

Immobilised animals

Test item conc.	Immobilised animals after
(mg/L)	3 h	6 h	24 h	48 h
nominal (test material)
0 (control)	0	0	0	0
0 (vehicle control)	0	0	0	0
0.78	0	0	0	0
1.56	0	0	0	0
3.13	0	0	0	15
6.25	1	1	11	20
12.5	2	2	20	20
25	18	19	20	20
50	20	20	20	20
100	20	20	20	20

pH and dissolved oxygen

Test item conc.	pH		Dissolved oxygen (mg/L)
(mg/L)	0 h	48 h	0 h	48 h
0 (control)	8.1	8.0	8.5	9.5
0 (vehicle control)	8.0	7.9	8.3	8.7
0.78	8.1	8.0	9.1	9.1
1.56	8.1	8.0	9.0	9.1
3.13	8.1	8.0	9.1	8.9
6.25	8.1	7.9	9.1	8.7
12.5	8.1	7.9	9.0	8.0
25	7.9	7.8	9.0	7.0
50	7.8	7.7	8.9	6.6
100	7.6	7.5	8.8	6.1

Effect concentrations

	nominal concentration based on	EC0	EC50	EC100
24 h	test material	3.13	6.16	12.50
	active ingredient	2.03	4.00	8.13
48 h	test material	1.56	3.85	6.25
	active ingredient	1.01	2.50	4.06

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of undecanal to aquatic invertebrates was 48 -h EC50: 2.5 mg/L and does not require classification for acute toxicity according to EU 286/2011, table 4.1.0

Executive summary:

The toxicity of undecanal was tested according to EC Directive 79/831/EEC which is similar to OECD TG 202. The test material was undecanal with a purity of 65%, therefore the effect concentrations were recalculated on the basis of the given purity. The test item was dissolved using Cremophor RH 40 as vehicle. Starting from a stock solution of 100 mg/L, a dilution series was prepared. The following concentrations were tested: nominal (active ingredient): 0 (control), 0 (solvent control), 0.51, 1.01, 2.03, 4.06, 8.12, 16.25, 32.5, and 65 mg/L. No analytical verification of the test concentrations was performed. Based on the volatilization half-life (lake: 5.2 d; see Ch. 5.4.4), a decrease of the test item concentration can be expected over the test duration, but most animals died within the first 24 h of the test.

The 48 -h EC50 was determined to be 3.85 mg/L based on test material and 2.5 mg/L based on active ingredient.

This result can be read across to n-/iso-undecanal. A detailed justification of read across is provided in the field Discussion of the endpoint summary of this section. It should be noted that the experimental effect concentration (48 -h EC50: 2.5 mg/L) is significantly higher than the LC50 value predicted by ECOSAR (0.863 mg/L, cf. endpoint summary).