Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

Toxicity to soil macroorganisms

Effects of 2,2'-dimethyl-4,4'-methylenebis(cyclohexylamine) (CAS 6864-37-5) on the reproducibility of earthworms (E. fetida) were studied in a GLP guideline study following OECD 222. Based on nominal concentrations, the EC10 for effects on reproduction was 228 mg/kg soil dw (95% CL: 115.6 / 450.8) (BASF, 2013; report No. 68E0695/00G009).

Toxicity to soil microorganisms

A nitrogen transformation test according to OECD 216 was performed under GLP to determine the effects of the test substance to soil microorganisms (BASF SE, 2013; project no. 37G0695/00X032). Negative effects on the nitrate formation were not found, therefore no effect concentrations could be calculated. Nitrate concentration in treated soil was significantly higher than the nitrate concentration of the solvent control soil at concentrations of greater than or equal to 62.5 mg/kg soil dw on day 0 and greater than or equal to 125 mg/soil dw on day 28 of the test.

The derived NOEC is based on the increase of nitrate in soil due to additional nitrate derived from the test substance: NOEC = 62.5 mg/kg soil, negative effects were lacking at the tested concentrations. It is assumed that the increase in nitrate content was due to the amount of nitrate entering the soil via the test substance.

A 28-d EC10 greater than 1000 mg/kg will be used for the environmental risk assessment.