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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

Mn(II)-IDHA_OECD203_Oncorhynchus mykiss: LC50 (96h) > 100 mg/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information

The acute toxicity of MnIDHA towards Rainbow trout was investigated according to OECD Guideline 203 in compliance with GLP (Konfederak, 2013). In this static test, Oncorhynchus mykiss was exposed to the test item at the limit test concentration of 100.0 mg/L and a control (0.0 mg/L). The exposure took place in glass aquaria with a capacity of 10 L. There was one replicate of the test concentration and the control. Ten fish were introduced into each aquarium. The fish were observed for mortality and intoxication symptoms after 3, 6, 24, 48, 72, and 96 hours of the exposure. All fish in the control and in the test concentration survived the 96-hour exposure. They did not exhibit any intoxication symptoms.

Chemical analyses were performed using a validated method. Samples collected at the exposure initiation (t0) and at the exposure termination (t96) were analyzed. The average concentration of MnIDHA in samples collected at the exposure initiation (t0) was 96.8% of the nominal concentration. The average concentration of MnIDHA in samples collected at the exposure termination (t96) was 84.1% of the initial concentration. Since the concentration of MnIDHA in the solution was higher than 80% of the initial concentration after 96 hours, the LCx values were calculated on the basis of the nominal concentration.

The LC50 values after 24, 48, 72, and 96 hours of the exposure determined on the basis of the nominal concentration of the test item are higher than 100.0 mg/L. The NOEC (96h) value is reported as≥100 mg/L based on the nominal concentration.