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EC number: 695-977-9
CAS number: 1309955-79-0
Effects of Bis (2-hydroxyethyl) oleyl
amine (CAS No 25307-17-9)(Batch no. S001018) on mortality, biomass and
the reproductive potential of the earthworm species Eisenia fetida(Savigny)
were determined according to the guidelines DIN ISO 11268-2 (1998), OECD
222 (2004) and BBA (1994) from July 17th to September 15th,
2009 at Dr.U.Noack-Laboratorienin
31157 Sarstedt, Germany. The study was conducted under static conditions
over 8 weeks with five different application rates (62.5 – 125 – 250 –
500 – 1000 mg/kg soil dry weight), which were mixed into natural soil.
Eighty sexually mature earthworms were inserted into 8 control
replicates (10 earthworms per replicate) and forty sexually mature
earthworms were divided into 4 replicates for the test item
concentrations. They had an individual body weight between 0.30 and 0.60
28 days no mortality was seen in the control and at all tested
application rates (Table 1). Also, no evident pathological symptoms or
changes in the behaviour of adult earthworms were seen in the control
and at all tested application rates. The
earthworm biomass increased in the control and at all tested application
rates over 28 days without any statistically significant differences at
62.5, 125, 250 and 500 mg/kg soil dry weight compared to the control.
However, at 1000 mg/kg soil dry weight the biomass increase was
statistically significant reduced compared to the control. After
further four weeks the reproduction rate (average number of juveniles)
was 332 in the control
and ranged between 254 and 359 at the test item application rates.
Compared to the control, the reproduction rate was statistically
significantly reduced at 1000 mg/kg soil dry weight. All other tested
application rates showed no statistically significant reduction compared
to the control (Table
the NOEC of Bis
(2-hydroxyethyl) oleyl amine (CAS No 25307-17-9) concerning
mortality, biomass and reproduction of earthworms was determined to be
at 500 mg/kg soil dry weight (Table 2).
validity criteria recommended by the test guidelines were fulfilled.
Summary of All Observed Effects in
the Tested Doses
Bis (2-hydroxyethyl) oleyl amine (CAS No 25307-17-9)
mg/kg soil dry weight
Mortality of adult earthworms
Reduction of body weight
Reduced reproduction rate
(Number of juveniles)
Summary of all effects
and LOEC for Reproduction, Mortality and Body Weight
NOECreproduction, mortality, body weight
LOECreproduction, mortality, body weight
No terrestrial test have been performed with di (2-hydroxypropyl)tallow amine. The data available for di (2-hydroxyethyl) oleyl amine (CAS No 25307-17-9) will as a worst-case be used for read-across. Justification for this read-across is presented in IUCLID chapter 13. For Di (2-hydroxyethyl) oleyl amine (CAS No 25307-17-9) one chronic earthworm test has been performed resulting in a NOEC of 500 mg/kg dw.
effects observed in a chronic terrestrial test up to 500 mg/kg dw.
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