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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2005
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report date:
2005

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
Deviations:
yes
Remarks:
The study integritywat not adversely affected by the deviation
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
yes
Remarks:
The study integritywat not adversely affected by the deviation
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
The study integritywat not adversely affected by the deviation
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
466-280-5
EC Name:
-
Cas Number:
67643-70-3
Molecular formula:
C13H23NO2
IUPAC Name:
3,7-dimethylocta-1,6-dien-3-yl N,N-dimethylcarbamate
Details on test material:
Batch 9000575061
Purity: 97.4%

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Stock solution were daily prepared in Acetoneat a factor of 10,000 higher than the final target concentrations.No special treatment other than a short period of shaking was applied to completely dissolve the stocks. Stocks were maintained homogeneous by continous stirring during the test period and were protected from light. These stocks are used for dosing.

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
5.7 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48h-EC50 based on analytically confirmed taget concentations was 5.6 mg/l with 95% confidence interval between 4.9 and 6.6 mg/l.