Reaction product of ammonium molybdate and C12-C24-diethoxylated alkylamine (1:5-1:3)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21-06-1993 to 30-07-1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP compliant guideline study

Data source

Reference

Reference Type:

other: Body responsible for the test

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Reaction product of ammonium molybdate and C12-C24-diethoxylated alkylamine (1:5-1:3)

EC Number:

412-780-3

EC Name:

Reaction product of ammonium molybdate and C12-C24-diethoxylated alkylamine (1:5-1:3)

Cas Number:

1380226-46-9

Molecular formula:

A complex mixture of species so no unique molecular formula can be given

IUPAC Name:

dimolybdenum(6+) diammonium 2-{2-[(octadec-9-en-1-yl)amino]ethoxy}ethan-1-ol heptaoxidandiide

Test material form:

liquid: viscous

Details on test material:

- Name of test material (as cited in study report): ADDITIV 104-KONZENTRAT
- Physical state: Liquid (dark red)
- Analytical purity: Product by process, treated as 100% pure
- Lot/batch No.: DS 147
- Expiration date of the lot/batch: March 9th, 1994
- Storage condition of test material: Room temperature in the dark

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No data reported

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Aliquots taken from each of the test concentrations

Test solutions

Vehicle:

no

Details on test solutions:

Weighed amounts of the test substance were spread out on microscope slides. The actual amounts weighed were 49.8, 91.2, 160.7, 280.9 and 501.1 mg. Each slide with test substance was put in 5 liters of ISO-medium. These solutions were stirred for Ca. 67 hours until the start of the exposure.
After stirring, the slides were removed. Then the resulting solutions (=Water Accommodated Fractions (WAF's)) were tested.

The final test solutions ranged from clear to turbid, with test substance particles deposited on the bottom of' the vessel containing the highest test substance concentration.

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Carp
- Source: Zodiac, proefacc, "De Haar Vissen", L.U. Wageningen, the Netherlands
- Mean length: 3.7 ± 0.2 cm
- Mean weight: 0.54 ± 0.07 g
- Feeding during test: No feeding from 24 hours prior to the test and during the total test period.

ACCLIMATION
- Acclimation period: At least 12 days following delivery
- Acclimation conditions (same as test or not): Following a 48-hour settling-in period the fish were allowed to acclimatise to the test medium without test substance for at least seven days.
- Type of food and feeding frequency: Daily with Trouvit or Artemia
- Health during acclimation (any mortality observed): In the batch of fish used for the test, mortality during the seven days prior to the start of the test was less than 5%.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Post exposure observation period:

The fish were observed at 3, 24, 48, 72 and 96 hours post exposure.

Test conditions

Hardness:

Dilution water: 250 mg CaCO3/L

Test temperature:

The temperature was measured daily in one control vessel, beginning at the start of the test. The temperature of the test medium measured in the control vessel ranged from 21.0 to 22.0°C

pH:

The pH was measured daily in all vessels, beginning at the start of the test (day 0). The pH during the test ranged from 7.8 to 8.1

Dissolved oxygen:

The dissolved oxygen content was measured daily in all vessels, beginning at the start of the test (day 0). The dissolved oxygen concentrations during the test ranged from 8.3 to 8.6 mg O2/l

Salinity:

No data reported

Nominal and measured concentrations:

A range-finding test was performed to provide information about the range of concentrations to be used in the final test. 3 fish per concentration were exposed to the Water Acommodated Fractions (WAFs) of 10 and 100 mg/l. In the range-finding test 100% mortality of fish was recorded in the WAF prepared at 100 mg/l. No mortality was seen in the WAF prepared at 10 mg/l. Based on the results of the range-finding test: WAF's prepared at 10, 18, 32, 56 and 100 mg/l were used for the definitive test

Details on test conditions:

TEST SYSTEM
- Test vessel: 8 litres, all glass
- Aeration: The test media were aerated continuously
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.76g fish/litre i.e. 7 fish per 5 litres of test medium

TEST MEDIUM / WATER PARAMETERS
ISO-medium, formulated using Milli-Ro water (tap—water purified by reverse osmosis; Millipore Corp., Bedford, Mass., USA) with the following composition:
Ca2 80 mg/l
Mg2 12 mg/l
Na 15 mg/l
K 3 mg/l
Cl 145 mg/l
SO42 49 mg/l
HCO3 47 mg/l
This dilution water was aerated until the dissolved oxygen concentration had reached saturation and the pH had stabilized. After aeration the hardness was 250 mg CaCO3 per litre and the pH 7.9.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily

Reference substance (positive control):

yes

Remarks:

RCC NOTOX PCKA-22 (Batch 93-04) 96-hour acute toxicity study in the carp with pentachiorophenol. June, 1993

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 10 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 10 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 10 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

> 10 mg/L

Details on results:

The 96h-LC5O for carp exposed to ADDITIV 104-KONZENTRAT was estimated to be between 10 mg/l (WAF prepared at 56 mg/l) and 20 mg/l (WAF prepared at 100 mg/l).

Results with reference substance (positive control):

The 96h-LC5O of this test is 0.34 mg/l with a 95% confidence interval between 0.30 and 0.42 mg/l. The historical range of the 96h-LC5O for carp recorded in reference tests approximately every 3 months since April 1988 is between 0.18 and 1.0 mg/l.

Reported statistics and error estimates:

No data recorded

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of both the range-finding test and the final study, ADDITIV 104- KONZENTRAT induced acute mortality when carp were exposed to a WAF prepared at 100 mg/L, containing an estimated ADDITIV 104-KONZENTRAT concentration of 20 mg/l. The 96h-LC5O for carp exposed to ADDITIV 104-KONZENTRAT was estimated to be between 10 mg/L (WAF prepared at 56 mg/L) and 20 mg/L (WAF prepared at 100 mg/l),with 0% mortality at 10 mg/L and 100% mortality at 20 mg/L. Since ADDITIV 104-KONZENTRAT was considered to be insoLubie in water and the concentrations in the WAF's were based on indirect measurements, the estimated concentrations have only limited relevance to the actual exposure
concentrations.

Executive summary:

In a GLP compliant, guideline 96 hr acute toxicity test the test substance induced acute mortality when carp were exposed to a WAF prepared at 100 mg/l, containing an estimated test substance concentration of 20 mg/l. The 96h LC5O for carp exposed to the test substance was estimated to be between 10 mg/l (WAF prepared at 56 mg/l) and 20 mg/l (WAF prepared at 100 mg/l), with 0% mortality at 10 mg/l and 100% mortality at 20 mg/l. Since the terst substance was considered to be insoiubie in water and the concentrations in the WAF's were based on indirect measurements, the estimated concentrations have only limited relevance to the actual exposure concentrations.