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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Aug - Sep 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
Samples of each test concentration were drawn from the approximate center of the test vessels. They were taken immediately before the exposure
and after 96 hours exposure and kept at -18°C to -22°c until analysis.
The oxygen content, pH and temperature were measured at 0, 24, 48, 72 and 96 hours.
Vehicle:
yes
Remarks:
86 .4 mg DMF (N ,N-dimethylformamide) and 3. 6 mg polyoxyethylen-sorbitan-monooleate (TWEEN 80) (FLUKA 93781) per liter
Details on test solutions:
Nominal : 4.0, 8.7, 19, 41, 90 mg test substance/I
Blanc: Dechlorinated tap water
Test organisms (species):
Cyprinus carpio
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Test temperature:
24 - 25°C
pH:
7.7 - 8.4
Dissolved oxygen:
73 - 107%
Nominal and measured concentrations:
Nominal: 4, 8.7, 19, 41, 90 mg/l
Details on test conditions:
Conc. Measured concentrations
nominal initial (Oh) end (96h) mean
mg/I mg/I % mg/I % mg/I %
Vehicle <0.1 <0.1 <0.1
4.0 3.7 93 3.7 93 3.7 93
8.7 8.1 93 8.2 94 8.1 93
19 17.8 94 17.7 93 17.7 93
41 36.3 89 37.1 90 36.7 89
90 85.9 95 79.8 89 82.8 92
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
16 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality (fish)
Remarks on result:
other: Values graphically determined
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Values calculated
Key result
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Values calculated
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
20 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Values calculated
Key result
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
23 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Values calculated
Key result
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
8.7 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality (fish)
Remarks on result:
other: Values observed
Key result
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
41 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality (fish)
Remarks on result:
other: Values observed
Validity criteria fulfilled:
yes
Conclusions:
Values calculated
LC 50 (96 h) 16 mg/I
95 % confidence limit 9.1 - 22 mg/l
Goodness of fit CHI 1.52

LC 50 (72 h) 16 mg/l
95 % confidence limit 9.1 - 22 mg/l
Goodness of fit CHI 1.52

LC 50 (48 h) 20 mg/I
95 % confidence limit 14 - 29 mg/l
Goodness of fit CHI 0.06

LC 50 (24 h) 23 mg/I
95 % confidence limit 16 - 40 mg/l
Goodness of fit CHI 1. 61

Values graphically determined
LC 50 (96 h) 16 mg/I

Values observed
NOEC (96 h) 8. 7 mg/I
LC O (96 h) 8. 7 mg/I
LC 100 (96 h) 41 mg/I

Controls
Mortalities in blank 0 %
Mortalities in vehicle 0 %

Description of key information

Values calculated

LC 50 (96 h)              16 mg/l

95 % confidence limit       9.1 - 22 mg/l

Goodness of fit CHI       1.52

LC 50 (72 h)              16 mg/l

95 % confidence limit       9.1 - 22 mg/l

Goodness of fit CHI       1.52

LC 50 (48 h)              20 mg/l

95 % confidence limit       14 - 29 mg/l

Goodness of fit CHI       0.06

LC 50 (24 h)              23 mg/l

95 % confidence limit       16 - 40 mg/l

Goodness of fit CHI       1. 61

Values graphically determined

LC 50 (96 h)              16 mg/l

Values observed

NOEC (96 h)              8. 7 mg/l

LC O (96 h)              8. 7 mg/l

LC 100 (96 h)       41 mg/l

Controls

Mortalities in blank       0 %

Mortalities in vehicle       0 %

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
16 mg/L

Additional information