Registration Dossier
Registration Dossier
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EC number: 604-047-3 | CAS number: 137863-20-8
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 7.7.1994 - 12.8.1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Composite samples of each test concentration were drawn by mixing identical volumes of the test solutions taken from of the test vessels. They were taken immediately before exposure and after 72 hours exposure and kept at - 18°C to - 22°C until analysis.
- Vehicle:
- yes
- Remarks:
- 99 mg N,N-dimethylformamide (DMF)/l and 1.0 mg polyoxy-ethylene- sorbitan-monooleate (TWEEN 90)/l
- Details on test solutions:
- Nominal: 4.4, 9.6, 21, 46 and 100 mg test substance/l
Actual: 2.1, 5.75, 11.5, 26.7 and 56 mg test substance/l - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- 10200 cells/ml
Collection of algae cultures
Pflanzenphysiologisches Institut
University
Nikolausberger Weg 18
D-3400 Gottingen
Germany - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23 °C
- pH:
- 7.8 - 8.8
- Nominal and measured concentrations:
- Nominal test concentrations: control, 4.4, 9.6, 21, 46, 100 mg/L
Measured test concentration: -, 2.1, 5.75, 11.5, 26.7, 56 mg/L - Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 56 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 56 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Validity criteria fulfilled:
- yes
- Conclusions:
- According to the classification criteria described in REGULATION (EC) No 1272/2008 (CLP) the product has not to be classified.
Values calculated
EC 50 (0-72 h) >56 mg/1
95 % confidence limit none
NOEC (0-72 h) (5 % level) 56 mg/1
Values observed
none
Reference
Description of key information
According to the classification criteria described in REGULATION (EC) No 1272/2008 (CLP) the product has not to be classified.
Values calculated
EC 50 (0-72 h) >56 mg/1
95 % confidence limit none
NOEC (0-72 h) (5 % level) 56 mg/1
Values observed
none
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 56 mg/L
- EC10 or NOEC for freshwater algae:
- 56 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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