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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
July - Octobet 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
Composite samples of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken immediately before exposure and after 48 hours exposure and kept at - 18°C to - 22°c until analysis.
Vehicle:
yes
Remarks:
30.7 mg N,N-dimethylformamide (DMF)/l and 1.3 mg polyoxy- ethylene-sorbitan-monooleate (TWEEN 80) /l per liter
Details on test solutions:
Nominal: 1.8, 3.2, 5.8, 10, 18 and 32 mg test substance/I
Calculated amounts of the stock solution to produce the desired test concentrations and vehicle to achieve identical vehicle concentrations were given into the water and were homogeneously distributed. The daphnia were then transferred into the beakers. The test substance was homogeneously distributed in the test vessels at all test times and test concentrations.
Test organisms (species):
Daphnia magna
Details on test organisms:
No. of Daphnia: 20 per concentration and control. 4 replicates of 5 daphnia each.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
240 mg CaC03/l
Test temperature:
21 - 22 °C
pH:
7.9
Dissolved oxygen:
99 - 103
Nominal and measured concentrations:
Nominal test concentrations: control, 1.8, 3.2, 5.8, 10, 18, 32 mg/L
Mean recovery rates: 88-95%
Key result
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
22 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
not specified
Conclusions:
Values calculated
EC 50 (48 h) 22 mg/l
95 % confidence limit 19 - 27 mg/l
Goodness of fit CHI 4.36

EC 50 (24 h) 24 mg/l
95 % confidence limit 21 - 29 mg/l
Goodness of fit CHI 1. 26

Values graphically determined
EC 50 ( 48 h ) 22 mg/l

Values observed
NOEC ( 48 h ) 10 mg/l
EC O ( 48 h ) 10 mg/l
EC 100 ( 48 h ) 32 mg/l

Controls
Immobilizations in blank 0
Immobilizations in vehicle 0

Description of key information

Values calculated

EC 50 (48 h)              22 mg/l

95 % confidence limit       19 - 27 mg/l

Goodness of fit CHI       4.36

EC 50 (24 h)              24 mg/l

95 % confidence limit       21 - 29 mg/l

Goodness of fit CHI       1. 26

Values graphically determined

EC 50 ( 48 h )       22 mg/l

Values observed

NOEC ( 48 h )       10 mg/l

EC O ( 48 h )              10 mg/l

EC 100 ( 48 h )       32 mg/l

Controls

Immobilizations in blank       0

Immobilizations in vehicle       0

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
22 mg/L

Additional information