Registration Dossier
Registration Dossier
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EC number: 604-047-3 | CAS number: 137863-20-8
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jan - Feb 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- The respiration rate of an activated sludge fed with a standard amount of synthetic sewage feed was measured after a contact time of 3 hours. The respiration rate of the same activated sludge in the presence of various concentrations of the test substance under otherwise identical conditions was also measured. The inhibitory effect of the test substance at a particular concentration was expressed as a percentage of the mean respiration rates of two controls. If possible, an IC 50 value was calculated from the inhibition values at the different concentrations.
- Vehicle:
- yes
- Details on test solutions:
- Stock solution 500 mg/I reference substance in deionized water.
Test concentrations 329, 103, 33 and 12 mg/I of the test substance. Because the test substance was not completely soluble in the test medium it was given directly into the test system without preparation of a stock solution.
32, 10 and 3.2 mg/I of the reference substance. - Test organisms (species):
- activated sludge
- Test type:
- not specified
- Water media type:
- freshwater
- Total exposure duration:
- 3 h
- Test temperature:
- 18 - 22°C
- pH:
- 8.0 - 8.3
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 329 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Due to its physicochemical properties the test substance was not completely soluble in the test medium. The reported results are related to the nominal concentration given into the test system.
- Results with reference substance (positive control):
- The IC50 (3 h) of the reference substance 3,5-Dichlorophenol was 11 mg/I
- Validity criteria fulfilled:
- yes
- Conclusions:
- The IC50 (3 h) of the test substance PBS 859 DS was > 329 mg/I
The IC20 (3 h) of the test substance PBS 859 DS was 95 mg/I
Reference
Description of key information
The IC50 (3 h) of the test substance PBS 859 DS was > 329 mg/l
The IC20 (3 h) of the test substance PBS 859 DS was 95 mg/l
Key value for chemical safety assessment
- EC50 for microorganisms:
- 329 mg/L
Additional information
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