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EC number: 619-057-3 | CAS number: 94667-33-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- fish early-life stage toxicity
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14/07/2000- 18/12/2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable.
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Samples to determine the concentration of test substance were taken weekly from each solution of the flow-through system. Temperature was measured continuously until day 26, then three times per week until day 34. pH and oxygen concentration were measured weekly.
- Vehicle:
- no
- Details on test solutions:
- Six concentrations of the test subject were used in this study. DSWL-E was used as a control. Stock solutions were created by mixing appropriate amounts of the labelled and unlabelled test substance with ultrapure water. The ratio of labelled to unlabelled was 15%/85% in the 1.0, 3.2, 10 and 32 µg/l test solutions, and 1.5%/98.5% in the 100 and 320 µg/l test solutions. New stock solutions were prepared weekly and stored in a refrigerator until use. The dosed volume of stock solution was approximately 0.1 ml per litre of dilution water.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- -Test organism: Zebra fish (Brachydanio rerio)
- Source: Atlanta fish hatchery, Hellevoetsluis, Netherlands
- Feeding during test
- Food type: Rotifers and Artemi nauplii
- Amount: Fed in abundance.
- Frequency: Rotifers immediately after hatching and Artemi nauplii after 10 days. - Test type:
- other: intermittent flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 34 d
- Post exposure observation period:
- Observations were made daily until all eggs had hatched, then every three days.
- Hardness:
- Total hardness 238 mg/l as CaCO3
- Test temperature:
- 23.4 to 25.5°C
- pH:
- 7.7 - 8.2
- Dissolved oxygen:
- Lowest measured oxygen concentration 6.7 mg/L.
- Salinity:
- No information provided.
- Nominal and measured concentrations:
- 0, 1.0, 3.2, 10, 32, 100 and 320 µg/l
Average measured values were above 100% of nominal values. - Details on test conditions:
- The intermittent flow-through system was constructed according to Van Leeuwen et al. Fish were confined to 4 chambers each of volume ca. 272 ml.
4 replicates of 20 eggs/larvae were dosed per concentration. Test substance concentrations were 0, 1.0, 3.2, 10 and 32 µg/l (15:85 % labelled:non-labelled) and 100 and 320 µg/l (1.5:98.5% labelled:non-labelled). Each replicate compartment was supplied with ca. 250 ml newly prepared dose solution every 51 minutes. - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 34 d
- Dose descriptor:
- LC50
- Effect conc.:
- 81 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Hatching, mortality, morphological and behavioural development, body weight and body length
- Remarks on result:
- other: 70 – 93µg/l
- Duration:
- 34 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 32 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Hatching, mortality, morphological and behavioural development, body weight and body length
- Duration:
- 34 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 100 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Hatching, mortality, morphological and behavioural development, body weight and body length
- Details on results:
- The test substance had no effect on hatching. All fish died at a test substance concentration of 320 µg/L. At 100 µg/l fish were observed swimming on the surface. No other observations were made at any other test substance concentrations. No morphological observations were made. The test substance had no effect on body lengths. Body weights appeared to increase at 100 µg/l. The NOEC calculated from the data was 32 µg/l, the 34day LC50 was 81 µg/l and the LOEC was 100 µg/l.
- Results with reference substance (positive control):
- No information provided.
- Reported statistics and error estimates:
- The LC50 values and their interval values wee calculated by means of a parametric model. Statistical significance for mortality was determined with a binomial test at 95% significance level, combining the results of the quadruplicates. Statistical significance for growth was determined with the two-tailed Dunnett-test with a 95% and 99% significance level.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this study, the LC50 was determined to be 81 µg/l, the NOEC was determined to be 32 µg/l and the LOEC was determined to be 100 µg/l.
- Executive summary:
In a study conducted in accordance with OECD Guideline No. 210, Zebra fish (Brachydanio rerio) were exposed to the test material, Bardac 22 (Didecyldimethylammonium Chloride), for 34 days at concentrations of 0, 1.0, 3.2, 10, 32, 100 and 320 mg/l. 4 replicates of 20 eggs/larvae were dosed per concentration. Hatching, mortality, morphological and behavioural development, body weight and body length were the test parameters examined.
The test substance had no effect on hatching. All fish died at a test substance concentration of 320 µg/l. At 100 µg/l fish were observed swimming on the surface. No other observations were made at any other test substance concentrations. No morphological observations were made. The test substance had no effect on body lengths. Body weights appeared to increase at 100 µg/l.
Under the conditions of this study, the 34-day LC50was determined to be 81 µg/l, the NOEC was determined to be 32 µg/l and the LOEC was determined to be 100 µg/l.
Reference
Table 1. Actual test substance concentration (% dosed amount of radioactivity)
Day |
Mean % radioactivity |
0 |
117.3 |
7 |
101.0 |
14 |
106.9 |
21 |
110.3 |
28 |
135.3 |
34 |
120.6 |
Overall mean |
115.2 |
Table 2. Mortality data
Dose concentration (mg/l) |
% eggs hatched after 6 days |
% mortality after 34 days |
0 |
100 |
2.5 |
1.0 |
100 |
8.8 |
3.2 |
100 |
1.2 |
10 |
100 |
5.0 |
32 |
100 |
6.2 |
100 |
100 |
66 |
320 |
100 |
100 |
Table 3. Growth of eggs/larvae of fish exposed to test substance
Dose concentration (mg/l) |
No. fish |
Mean final length (cm) |
Mean final dry weight (mg) |
0 |
78 |
1.32±0.17 |
1.83±0.09 |
1.0 |
73 |
1.36±0.15 |
2.32±0.44 |
3.2 |
79 |
1.30±0.14 |
1.96±0.18 |
10 |
76 |
1.31±0.19 |
2.31±0.83 |
32 |
75 |
1.34±0.15 |
2.11±0.27 |
100 |
27 |
1.30±0.25 |
3.49±1.71 |
320 |
0 |
n/d |
n/d |
n/d no data (all fish had died)
Description of key information
One key study is available. The study was conducted in accordance with OECD guideline 210, with no deviations and compliant with GLP. The species tested was Brachydanio rerio (zebra fish), which were exposed to Bardac 22 (read-across from structural analog DDAC) for 34 days. Under the conditions of this study, the 34-day LC50was determined to be 81 µg/L, the NOEC was determined to be 32 µg/L and the LOEC was determined to be 100µg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 32 µg/L
Additional information
In a study conducted in accordance with OECD Guideline No. 210, Zebra fish (Brachydanio rerio) were exposed to the test material, Bardac 22 (Didecyldimethylammonium Chloride), for 34 days at concentrations of 0, 1.0, 3.2, 10, 32, 100 and 320mg/l. 4 replicates of 20 eggs/larvae were dosed per concentration. Hatching, mortality, morphological and behavioural development, body weight and body length were the test parameters examined. The test substance had no effect on hatching. All fish died at a test substance concentration of 320 µg/l. At 100 µg/l fish were observed swimming on the surface. No other observations were made at any other test substance concentrations. No morphological observations were made. The test substance had no effect on body lengths. Body weights appeared to increase at 100 µg/l.
Under the conditions of this study, the 34-day LC50was determined to be 81 µg/l, the NOEC was determined to be 32 µg/l and the LOEC was determined to be 100µg/l.
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