Linseed oil, oxidized

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 1988 - July 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Not GLP, according to standard test method, but not in agreement with OECD 209.

Qualifier:

according to guideline

Guideline:

other: B. Robra, Wasser/Abwasser 117, 80 (1976)

Deviations:

no

Principles of method if other than guideline:

Pseudomonas putida cultures are exposed to the test substance for a period of 30 min to 3 hours. The oxygen consumption (in terms of mg/L/hour) is then measured and compared to that of the control. The percentage inhibition of the oxygen consumption is measured and EC values are estimated.

GLP compliance:

no

Analytical monitoring:

no

Details on sampling:

- Sampling method: not relevant, during this test the oxygen consumption is measured using e.g. an oxygen electrode

Vehicle:

yes

Details on test solutions:

The test solutions were prepared using an emulsifying agent: Laurylmyristylalcoholethoxylate 7 E.O

Test organisms (species):

Pseudomonas putida

Test type:

static

Water media type:

not specified

Limit test:

no

Remarks on exposure duration:

no data, the guideline prescribes a period of 30 min to 3 hours

Hardness:

not relevant

Test temperature:

no data

pH:

no data

Dissolved oxygen:

no data

Salinity:

no data

Nominal and measured concentrations:

Nominal concentrations: 80000; 90000; 95000 and 100000 mg/L
The concentrations were not verified by chemical analysis.

Details on test conditions:

TEST SYSTEM
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): not reported
- No. of vessels per vehicle control (replicates): not reported
no further data available

EFFECT PARAMETERS MEASURED (with observation intervals if applicable)
Oxygen consumption was measured, the inhibition of oxygen consumption was calculated and the EC10 was determined

Reference substance (positive control):

no

Dose descriptor:

EC0

Effect conc.:

80 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: no data on the duration, the guideline prescribes a period of 30 min to 3 hours

Dose descriptor:

EC10

Effect conc.:

90 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: no data on the duration, the guideline prescribes a period of 30 min to 3 hours; Based on the mean of 2 replicates

Dose descriptor:

EC50

Effect conc.:

> 100 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: no data on the duration, the guideline prescribes a period of 30 min to 3 hours; Based on the mean of 2 replicates

Details on results:

- Effect concentrations exceeding solubility of substance in test medium: yes, because the solubility of the test material in water is <1 mg/L. However, an emulsifying agent was used and the guideline prescribes that the test solutions are aerated during the test, which enhances mixing.

No data on the duration, the guideline prescribes a period of 30 min to 3 hours

Validity criteria fulfilled:

not specified

Conclusions:

Single species test with Pseudomonas putida:
EC10: 90 g/L
EC50: > 100 g/L

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 1988 - July 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Not GLP, according to standard test method, but not in agreement with OECD 209.

Qualifier:

according to guideline

Guideline:

other: B. Robra, Wasser/Abwasser 117, 80 (1976)

Deviations:

no

Principles of method if other than guideline:

Pseudomonas putida cultures are exposed to the test substance for a period of 30 min to 3 hours. The oxygen consumption (in terms of mg/L/hour) is then measured and compared to that of the control. The percentage inhibition of the oxygen consumption is measured and EC values are estimated.

GLP compliance:

no

Analytical monitoring:

no

Details on sampling:

- Sampling method: not relevant, during this test the oxygen consumption is measured using e.g. an oxygen electrode

Vehicle:

yes

Details on test solutions:

The test solutions were prepared using an emulsifying agent: Laurylmyristylalcoholethoxylate 7 E.O.

Test organisms (species):

Pseudomonas putida

Test type:

static

Water media type:

not specified

Limit test:

no

Remarks on exposure duration:

no data, the guideline prescribes a period of 30 min to 3 hours

Hardness:

no data

Test temperature:

no data

pH:

no data

Dissolved oxygen:

no data

Salinity:

no data

Nominal and measured concentrations:

Nominal concentrations: 5000; 10000; 30000; 40000; 50000 and 100000 mg/L
The concentrations were not verified by chemical analysis

Details on test conditions:

TEST SYSTEM
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): not reported
- No. of vessels per vehicle control (replicates): not reported
No further data available

EFFECT PARAMETERS MEASURED (with observation intervals if applicable)
Oxygen consumption was measured, the inhibition of oxygen consumption was calculated and the EC10 was determined

Reference substance (positive control):

no

Dose descriptor:

EC0

Effect conc.:

< 5 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: no data on the duration, the guideline prescribes a period of 30 min to 3 hours

Dose descriptor:

EC10

Effect conc.:

6 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: no data on the duration, the guideline prescribes a period of 30 min to 3 hours

Dose descriptor:

EC50

Effect conc.:

ca. 25 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: EC50 not reported; estimated by the author of this summary from the reported graph of % inhibition versus test concentration; no data on the duration, the guideline prescribes a period of 30 min to 3 hours

Details on results:

- Effect concentrations exceeding solubility of substance in test medium: yes, because the solubility of the test material in water is <1 mg/L. However, an emulsifying agent was used and the guideline prescribes that the test solutions are aerated during the test, which enhances mixing.

No data on the duration, the guideline prescribes a period of 30 min to 3 hours.

Validity criteria fulfilled:

not specified

Conclusions:

Single species test with Pseudomonas putida:
EC10: 6 g/L
EC50: ca. 25 g/L

Reference 3

Endpoint:

activated sludge respiration inhibition testing

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

the study does not need to be conducted because the substance is found to be readily biodegradable and the applied test concentrations are in the range of concentrations that can be expected in the influent of a sewage treatment plant

Description of key information

At a concentration of 15.5 mg/L in the OECD 301B study it was shown that the substance does not inhibit microbial activity.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

15.5 mg/L

Additional information

Two studies are available for Pseudomona putida. An emusifier is used to prepare the test solutions. The test substance that show toxic effects at extremely high concentrations with the lowest EC10 = 6 g/L and lowest EC50 = 25 g/L. This study is considered to be in line with OECD 209. However, a more representative value is derived from the biodegradation study (OECD 301B) where the substance did not inhibit the microbial activity at 15.5 mg/L.