3-((3,4-dicyanophenyl)sulfonyl)-N-(2-hydroxypropyl)propane-1-sulfonamide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Experimental start date 21 April 2017 Experimental completion date 05 May 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on test solutions:

Analysis of the freshly prepared bulk test preparations at 0 and 72 hours showed measured test concentrations to be 90 and 88mg/L. There was no significant change in the measured concentrations of the old or expired test media at 24 and 96 hours and so the results are based on nominal concentration.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

The test was carried out using juvenile rainbow trout. The stock fish were fed commercial trout pellets which was discontinued approximaelty 22.5 hours prior to the start of the definitive test. There was no mortality in the 7 days prior to the start of the test and the fish had a mean standard length of 5.5cm ( sd = 0.40) and a mean weight of 1.4g (sd = 0.4) at the end of the definitive test. Based on the mean weight vlaue this gave a loading rate of 0.48g bodyweight/liter ( static volume).

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

14-15°C

pH:

Fresh media control pH range 7.6 - 8.1
Nominal concentration 100 pH range 7.9 -8.2

Dissolved oxygen:

9.5mg O2/L

Nominal and measured concentrations:

Nominal concentration of 100mg/L was used.

Details on test conditions:

At the start of the test seven fish were placed in each test vessel at random, in the test preparations. The vessels were covered to reduce evaporation and maintained at 14-16°C with a photoperiod of 16 hours light and 8 hours darkness with a 20 minute dawn and dusk transition. The water temperature, pH and dissolved oxygen concentrations were recorded daily throughout the test, The measurements at 0 hours and after each test media renewal at 24, 48 and 72 hours, represent those of the freshly prepared test preparations. The pH and dissoved oxygen concentrationswere measured using a Hach flex handheld meter, the temperature was measured using a digital thermometer.

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

Sub lethal effects of exposure were observed at both the control and 100mg/L test concentrations. These responses inclduing sitting and swimming at the bottom of the tanks.

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of the test item to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and nominal test concentrations gave a 96-Hour LC50 of greater than 100 mg/L. The No Observed Effect Concentration was 100 mg/L.

Executive summary:

Introduction

This study was performed to assess the acute toxicity of the test item to rainbow trout. The method was designed to be compatible with the OECD guideline for testing of chemicals (1992) No 203 "Fish,acute toxicity test" referenced as Method C.1 of commission regulation (EC) no 440/2008.

Results and conclusion

Exposure of the rainbow trout to the test item gave LC50 values based on the nominal test concentrations of > than 100mg/L. The No Observed Effect Concentration was 100mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L