Esterification products of Grape seeds, Vitis vinifera L. (Vitaceae), extract with hexadecanoyl chloride

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

1

Reference substance name:

Esterification products of Grape seeds, Vitis vinifera L. (Vitaceae), extract with hexadecanoyl chloride

EC Number:

941-319-3

Molecular formula:

Unknown (Substance of Unknown or Variable Composition)

IUPAC Name:

Esterification products of Grape seeds, Vitis vinifera L. (Vitaceae), extract with hexadecanoyl chloride

Test material form:

solid: particulate/powder

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

ca. 28 d

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Key result

Remarks on result:

other: not readily biodegradable according to OECD 301B and EU-Method C.4-C

Applicant's summary and conclusion

Conclusions:

According to attached study report, the Therefore, the test item is not readily biodegradable within 28 days.

Executive summary:

The test item Palmitoyl grape seed extract was tested using a concentration of nominally 20 mg organic carbon/L (corresponding to 26.1 mg Palmitoyl grape seed extract/L) in test medium following OECD 301B and EU-Method C.4-C. Aniline was chosen as positive control. Activated sludge was used as inoculum (concentration in the test 25.0 mg dry matter/L). The test was left running for 28 days. All validity criteria were met. Degradation of the positive control was 64 % after 7 days. The following data were determined for the test item Palmitoyl grape seed extract: 10-day-window: not detected degradation at the end of 10-day-window no degradation degradation at the end of the test 0 % pass level following guideline: 60% at the end of 10-day-window for pure substances respective 60 % at the end of the test for mixtures Because the test item is a mixture, the 10-day-window has not to be taken into account. As degradation missed 60% in the course of the test, Palmitoyl grape seed extract is considered as not readily biodegradable.