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EC number: 206-982-9 | CAS number: 407-25-0
Endpoint summary
Administrative data
Description of key information
Additional information
Trifluoroacetic acid anhydride (TFAH) reacts violently with water and is instantaneously degraded in trifluoroacetic acid (TFA) which is the relevant substance for risk assessment and classification & labelling purposes.
Acute data of various reliability, some based on international guidelines, are available for three trophic levels: Algae, Invertebrates and Fish and are summarised in the table below. In aqueous solution, the pH of the substance is naturally low and for testing on organisms either the sodium salt (NaTFA) or pH adjustment were required.
Among all the species tested, a toxic effect (growth inhibition) was found only for the algae Selenastrum capricornutum. Three tests were performed on this species. However one of the studies was further disregarded because a higher pH observed in the control was probably responsible for lower toxicty (one order of magnitude difference) compared to the two others. Based on the geometric mean of the two remaining tests, an ErC50 of 8.5 mg/L was calculated.
Six other freshwater algae and three marine algae were tested and no toxicity was found on none of the species at the highest concentrations tested.
One acute study and one chronic study (reproduction test) are available on Daphnia magna to assess the toxicity of TFA on invertebrates. No short term nor long term toxicty was observed for Daphnia magna. The EC50 (48h) was greater than or equal to the highest concentration tested of 999 mg TFA/L and a chronic daphnid 21 days reproduction test performed up to a maximal concentration of 25 mg/L of TFA. While this value is taken as the NOEC for invertebrates it is not a true NOEC as no effect concentration was statistically calculated.
One acute study is available on Danio rerio and no toxicity was observed at the highest concentrations tested. Therefore, an LC50 (96h) > 999 mg/L TFA was derived for the fish.
Table: Acute and chronic toxicity of TFA on aquatic species
Organism |
EC50/LC50 (mg/L) |
NOEC (mg/L) |
Flag |
Reference in IUCLID 5 |
ACUTE |
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Aquatic algae |
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Freshwater |
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Selenastrum capricornutum |
Growth rate 72h:11.4(TFA) |
Growth rate72h*:0.10(TFA) |
K |
6.1.5 Alga tox., V2 1995 GROE |
Selenastrum capricornutum |
Growth rate 72h:6.4(TFA) |
Growth rate72h*:0.42(TFA) |
K |
6.1.5 Alga tox., V2 1996 THIE |
Selenastrum capricornutum |
Growth rate 72h:133(TFA) |
Growth rate 72h:0.30(TFA) |
D |
6.1.5 Alga tox., V2 1992 GROE |
Scenedesmus subspicatus |
Growth rate 72h:> 99.9(TFA) |
Growth rate72h*:99.9(TFA) |
K |
6.1.5 Alga tox., V2 1995 BERE |
Chlorella vulgaris |
Growth rate 72h:> 999(TFA) |
Growth rate 72h:999(TFA) |
K |
6.1.5 Alga tox., V1 1993 BERE |
Chlamydomonas reinhardtii |
Growth rate 72h:> 99.9(TFA) |
Growth rate 72h:99.9(TFA) |
K |
6.1.5 Alga tox., V1 1996 BERE GROE |
Microcystis aeruginosa |
Growth rate 144h:> 97(TFA) |
Growth rate 144h:97(TFA) |
K |
6.1.5 Alga tox., V2 1996 BERE 3 |
Navicula pelliculosa |
Growth rate 96h:1997(TFA) |
Growth rate 96h:499(TFA) |
K |
6.1.5 Alga tox., V2 1994 SMYT 1 |
Anabaena flos-aquae |
Growth rate 120h:> 1997(TFA) |
Growth rate 120h:499(TFA) |
K |
6.1.5 Alga tox., V1 1994 SMYT 3 |
Marine water |
||||
Dunaliella tertiolecta |
Growth rate 72h:> 103(TFA) |
Growth rate 72h:<103(TFA) |
K |
6.1.5 Alga tox., V1 1996 BERE 1 |
Phaeodactylum tricornutum |
Growth rate 72h:> 97(TFA) |
Growth rate 72h:97(TFA) |
K |
6.1.5 Alga tox., V1 1996 BERE 2 |
Skeletonema costatum |
Growth rate 96h:> 1997(TFA) |
Growth rate 96h:1997(TFA) |
K |
6.1.5 Alga tox., V1 1994 SMYT 2 |
Aquatic invertebrates |
||||
Daphnia magna |
48h:> 999(TFA) |
48h:999(TFA) |
K |
ST aq. Inv., V1 1992 GROE |
Fish |
||||
Brachydanio rerio |
96h:> 999(TFA) |
96h:≥ 999(TFA) |
K |
ST fish, V1 1992 GROE |
CHRONIC |
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Aquatic invertebrates |
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Daphnia magna |
21d:> 25(TFA) |
21d:≥ 25(TFA) |
K |
LT aq. Inv., V1 2010 KUHL |
Moreover, one key study is available to evaluated the influence of NaTFA on the activated sludge respiration rate. In this study, the 3 hours EC10, EC20 and EC50 could not be quantified because up to the highest nominal test concentration (1000 mg NaTFA/L, corresponding to 832 mg TFA/L) less than 10% inhibition was noted. Nevertheless, the 3-hour EC20 and EC50 are clearly higher than 832 mg TFA/L under the present test conditions. The NOEC/EC10 may be established above 832 mg TFA/L.
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