Sodium 2-[[2-amino-8-hydroxy-6-[(methylanilino)sulphonyl]-1-naphthyl]azo]-5-(chloroacetamido)benzenesulphonate

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

03 November 2014 to 07 November 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

GLP compliance:

no

Type of method:

flask method

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- lot/batch number of test material: AT-0027506800

Water solubility:

27.4 mg/L

Conc. based on:

test mat.

Temp.:

20 °C

Remarks on result:

other: pH is not available

Individual Results

 

Preliminary Visual Estimation of the Water Solubility

Table 1: Appearance of the test mixtures in the preliminary test

 

Amount of the test item in mg	Total volume in ml	Appearance of mixture
10	10	Undissolved
	50	Undissolved
	100	Undissolved

 

The preliminary visual experiments showed that the water solubility of the test item is below 100 mg/l.

 

Flask-Method

Table 2: Water solubility of the test item – experiments A to D

Experiment	A	B	C	D(blank)
Amount of test item(mg)	500	500	500	---
Stirring time at 30°C (h)	24	48	72	72
Aliquot	0.5/3 ml	0.5/3 ml	0.5/3 ml	0.5/3 ml
Measured concentration (μg/ml)	4.53	450	4.70	n.d.
Water solubility (mg/l)	27.2	27.0	28.0	---

Average experiments A to C: 27.4 mg/l

Conclusions:

The water solubility of FAT 20075/C was determined to 27.4 mg/l.

Executive summary:

The water solubility of FAT 20075/C was carried out according to OECD guideline 105 and EU method A.6 using flask method.

For the main test three flasks were prepared with about 500 mg test item and 4 ml water. The batches were stirred for different times at 30°C (experiment A for 24h , experiment B for 48h , experiment C for 72h ) in tightly closed flasks. After equilibration (further stirring for 24h at 20 °C) a saturated solution with a precipitate of the test item could be observed. Aliquots were taken and centrifuged (30 minutes with 5000 rpm at 20°C). Subsequently , the solution was filtered over 0,20 μm microfilters. The solution is yellow colored and undiluted analysed by VIS-spectroscopy (509 nm).

In the preliminary visual experiments showed that the water solubility of the test item is below 100 mg/l.

Based on the study results, the water solubility off FAT 20075/C at a temperature of 20°C was determined to (without correction for the purity) 27.4 mg/l.

Description of key information

The water solubility of FAT 20075/C was carried out according to OECD guideline 105 and EU method A.6 using flask method. For the main test three flasks were prepared with about 500 mg test item and 4 ml water. The batches were stirred for different times at 30 °C (experiment A for 24 h, experiment B for 48 h, experiment C for 72 h) in tightly closed flasks. After equilibration (further stirring for 24 h at 20 °C) a saturated solution with a precipitate of the test item could be observed. Aliquots were taken and centrifuged (30 minutes with 5000 rpm at 20 °C). Subsequently, the solution was filtered over 0.20 μm microfilters. The solution is yellow colored and undiluted analysed by VIS-spectroscopy (509 nm). In the preliminary visual experiments showed that the water solubility of the test item is below 100 mg/l. Based on the study results, the water solubility off FAT 20075/C at a temperature of 20 °C was determined to (without correction for the purity) 27.4 mg/l.

Key value for chemical safety assessment

Water solubility:

27.4 mg/L

at the temperature of:

20 °C