2-isopropyl-N-hydroxyethyl 1,3-oxazolidine;methyl carbonato-N-ethyl-2-isopropyl-1,3-oxazolidine;reaction mass of: carbonato-bis-N-ethyl-2-isopropyl-1,3-oxazolidine

Endpoint summary

Additional information

Short-term toxicity to fish

A 96-hour semi-static study was conducted according to OECD TG 203 and Directive 92/69/EEC method C.4 to determine the acute toxicity of Incozol LV to Oncorhynchus mykiss. The concentration range included the following nominal Incozol LV concentrations: 100, 50, 25, 12.5 and 6.3 mg/L. The test media were prepared by direct addition of Incozol LV to the test vessels, the test vessels were then fully filled, sealed and stirred for approximately 2 h. A control treatment was prepared containing dilution water only. Analytical samples were taken from each test medium to determine the concentration of the major breakdown product of Incozol LV. Analytical samples were taken at the start and end of the first and fourth exposure periods. The 96-h LC50 of Incozol LV to O. mykiss based on nominal exposure concentrations was calculated to be 85.7 mg/L. The 96-h NOEC was determined to be 50 mg/L.

Short-term toxicity to aquatic invertebrates

A study was conducted according to OECD TG 202 and Directive 92/69/EEC method C.2 to determine the acute toxicity of Incozol LV to aquatic invertebrates. The study was conducted as limit test. Therefore, a solution with concentration of 100 mg/L test item was prepared by direct addition of Incozol LV to dilution water; the test medium was stirred for approximately 2 h before addition to the test vessels. Following addition of the D. magna the test vessels were sealed. A control treatment was prepared containing dilution water only. No immobility of D. magna was observed, accordingly, the 24- and 48-h EC50 values of Incozol LV were determined to be > 100 mg/L.

Toxicity to aquatic algea

A study was conducted according to OECD TG 201 and Directive 92/69/EEC to determine the effects of Incozol LV on the alga Pseudokirchneriella subcapitata. A 72-hour sealed study was performed. Therefore, 100 mg/L stock solution was prepared by direct addition of Incozol LV to the algal nutrient medium and stirred for approximately 2 h. Aliquots of the stock solution were used and further diluted with algal nutrient medium to achieve the desired concentration series. A control treatment was prepared containing algal nutrient medium only. The test was conducted with 6 concentrations as follows: 100, 50, 25, 12.5, 6.3 and 3.2 mg/L. Two tests were performed, the definitive test using electronic particle counting and a confirmatory test which was conducted using a light microscope and a haemacytometer. Due to low initial cell inoculum it was not possible to perform counts using the light microscope and haemacytometer at 24 and 48 h, therefore, it was only possible to calculate a 72 h E(b)C50 based on the areas under the growth curves. Based on the areas under the growth curves the 72-h E(b)C50 of Incozol LV to Pseudokirchneriella subcapitata was observed to be 21.6 mg/L (with 95 % confidence limits of 11.9 to 47.6 mg/L). Based on the average specific growth rate the 72-h E(r)C50 of Incozol LV to P. subcapitata was observed to be 93.1 mg/L (with 95 % confidence limits of 74.2 to 126 mg/L). Based on the biomass the NOEC of Incozol LV was observed to be 3.2 mg/L. Based on the growth rate the NOEC of Incozol LV was observed to be 6.3 mg/L.

Toxicity to microorganism

A study was conducted according to OECD TG 209 and Directive 87/302/EEC part C, to asses the capacity of Incozol LV to inhibit the aerobic respiratory process of the assorted microorganisms present in activated sludge.Therefore samples of activated sludge were exposed to various nominal concentrations of Incozol LV, ranging from 1.0 to 1000 mg/L, and their respiration rates measured after 3 h contact time. There was no evidence of significant inhibition at any of these concentrations, and consequently no definitive experiment was performed. Incozol LV did not inhibit the respiration of activated sludge at concentrations up to and including 1000 mg/L. The concentration of Incozol LV causing a 50 % reduction in respiration rate, relative to untreated controls (EC50), could not be determined from the results of this study, but it exceeded 1000 mg/L.