Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 269-924-1 | CAS number: 68391-05-9 This substance is identified by SDA Substance Name: C12-C18 dialkyl dimethyl ammonium chloride and SDA Reporting Number: 16-047-00.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From February 11, 1998 to February 20, 1998
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
- Remarks:
- Study conducted according to ISO/CD 14669 and the PARCOM ring test protocol in compliance with GLP
- Qualifier:
- according to guideline
- Guideline:
- other: ISO/CD 14669 and the PARCOM ring test protocol
- Deviations:
- yes
- Remarks:
- pH and O2 measurement frequency slightly altered
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- Sampling not performed
- Vehicle:
- no
- Details on test solutions:
- - Dilution water used was natural seawater from the Eastern Scheldt. This water was checked for contaminants (volatile organics, organochlorine compounds and oil) before use.
- The water was also filtered and recirculated over a carbon filter before use. 0.0030 g of the test substance was dissolved directly in 3L of diluted natural seawater. From this 1mg/L stock the test concentrations were made.
- The substance was dispersable in water - Test organisms (species):
- other: aquatic crustacea: Acartia tonsa
- Details on test organisms:
- - The test animal was the marine crustacean Acartia tonsa cultured in the laboratory under standard conditions.
- The animals used in the test were copepodites (subadults) between 6 and 8 days old. - Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No, during the test only.
- Hardness:
- No data
- Test temperature:
- The temperature as measured in the control medium was 19.6 ⁰C at the start of the test and 19.4 ⁰C at the end
- pH:
- 8.0-8.2
- Dissolved oxygen:
- 7.3 - 7.7 mgO2/l
- Salinity:
- (32% diluted to) 29%
- Nominal and measured concentrations:
- Nominal: 0, 0.056, 0.10, 0.18, 0.32, 0.56, 1.0 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 10 small glass vessels
- Type (delete if not applicable): Closed
- No. of organisms per vessel: 1
- Material, size, headspace, fill volume: 10 mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Sea water
- Diluted seawater at 29% salinity was used in the control
OTHER TEST CONDITIONS
- Photoperiod: 16 h light: 8 h dark - Reference substance (positive control):
- no
- Key result
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 0.397 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 0.318-0.495 mg/L
- Remarks:
- equivalent to 0.298 mg a.i./L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 0.295 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 0.237-0.367 mg/L
- Remarks:
- equivalent to 0.221 mg a.i./L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 0.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality and behaviour
- Remarks on result:
- other: equivalent to 0.075 mg a.i./L
- Details on results:
- - Mortality and condition have been recorded in the test. Immobile, sluggish or drugged animals were not observed and no mortality in the controls.
- The LC50 and NOEC 48h were 0.295 and 0.10 mg/L respectively. - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Maximum likelihood estimates of the LC50 values were calculated assuming a log-logistic dose-effect relationship. Likelihood-ratio confidence intervals were derived from the confidence intervals.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the 24 h LC50, 48 h LC50 and 48 h NOEC of test substance to Acartia tonsa in diluted natural seawater were determined to be 0.397, 0.295 and 0.10 mg/L (equivalent to 0.298, 0.221 and 0.075 mg a.i./L), respectively.
- Executive summary:
A study was conducted to determine the acute toxicity of the test substance, C12-18 DAQ (75% active) to Acartia tonsa (Copepod: Crustacea), according to the draft International Standard ISO/CD 14669 and a PARCOM ring-test protocol in compliance with GLP. Ten individual replicate scintillation vials, each containing one Acartia tonsa were exposed to nominal concentrations of 0.056, 0.10, 0.18, 0.32, 0.56 and 1.0 mg/L for 48 h under static conditions. No chemical analyses of the test concentrations were performed during the test and all concentrations mentioned are nominal concentrations of the test substance. Maximum likelihood estimates of the LC50 values were calculated assuming a log-logistic dose-effect relationship. The results of the tests based on nominal concentrations were 24 h LC50 0.397 (0.318-0.495) mg/L, 48 h LC50 0.295 (0.237 -0.367) mg/L, 48 h NOEC (mortality and behaviour) 0.10 mg/L. At the start of the test all test media were clear and colorless (visually assessed). No mortality was observed among the control population following a 48 h exposure. Under the study conditions, the 48 h LC50 and NOEC of the test substance to Acartia tonsa in diluted natural seawater were determined to be 0.295 and 0.10 mg/L (equivalent to 0.221 and 0.075 mg a.i./L), respectively (Schoonmade, 1998).
Reference
Results
Table 1 Summary of the data on survival in the acute toxicity test with Acartia tonsa exposed to test material
Time (h) |
Nominal concentration of test substance (mg/L) |
||||||
0 |
0.056 |
0.10 |
0.18 |
0.32 |
0.56 |
1.0 |
|
0 |
10 |
10 |
10 |
10 |
10 |
10 |
10 |
24 |
10 |
10 |
10 |
10 |
8 |
1 |
0 |
48 |
10 |
10 |
10 |
8 |
6 |
0 |
0 |
Description of key information
Based on the study result, the 48 h LC50 of the test substance to Acartia tonsa in diluted natural seawater was determined to be 0.221 mg a.i./L (nominal).
Key value for chemical safety assessment
Marine water invertebrates
Marine water invertebrates
- Dose descriptor:
- LC50
- Effect concentration:
- 0.22 mg/L
Additional information
A study was conducted to determine the acute toxicity of the test substance, C12-18 DAQ (75% active) to Acartia tonsa (Copepod: Crustacea), according to the draft International Standard ISO/CD 14669 and a PARCOM ring-test protocol in compliance with GLP. Ten individual replicate scintillation vials, each containing one Acartia tonsa were exposed to nominal concentrations of 0.056, 0.10, 0.18, 0.32, 0.56 and 1.0 mg/L for 48 h under static conditions. No chemical analyses of the test concentrations were performed during the test and all concentrations mentioned are nominal concentrations of the test substance. Maximum likelihood estimates of the LC50 values were calculated assuming a log-logistic dose-effect relationship. The results of the tests based on nominal concentrations were 24 h LC50 0.397 (0.318-0.495) mg/L, 48 h LC50 0.295 (0.237 -0.367) mg/L, 48 h NOEC (mortality and behaviour) 0.10 mg/L. At the start of the test all test media were clear and colorless (visually assessed). No mortality was observed among the control population following a 48 h exposure. Under the study conditions, the 48 h LC50 and NOEC of the test substance to Acartia tonsa in diluted natural seawater were determined to be 0.295 and 0.10 mg/L (equivalent to 0.221 and 0.075 mg a.i./L), respectively (Schoonmade, 1998).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

EU Privacy Disclaimer
This website uses cookies to ensure you get the best experience on our websites.