Glycerides, C16-18 mono-, di- and tri-, hydrogenated, citrates, sodium salts

Toxicity to microorganisms

Description of key information

No data are available for CAS 91744-39-7. Thus, read-across from the analogue substance CAS 91744-38-6 is used.
The NOEC of the test item Glyceryl Stearate Citrate (Glycerides, C16-18 mono-, di-, and tri-, hydrogenated, citrates, potassium salts) is 1000 mg/L.
The test item is not toxic at the limit concentration of 1000 mg/L to activated sludge of a municipal sewage treatment plant.

Additional information

A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with the test item Glyceryl Stearate Citrate (Glycerides, C16-18 mono-, di-, and tri-, hydrogenated, citrates, potassium salts; CAS 91744-38-6) (batch number: 105618) from 2012-06-11 to 2012-06-12, with the definitive experimental phase on 2012-06-12 at Dr. U.Noack-Laboratorien, 31157 Sarstedt, Germany. Test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany. The test was carried out under static conditions with the limit test item concentration of 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates ranged from -16 % to -22 %.

In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC₅₀-value for the reference item was 99.1 mg/L.

Table 1:    NOEC-Value of Glyceryl StearateCitrate (Glycerides, C16-18 mono-, di-, and tri-, hydrogenated, citrates, potassium salts)

		Test Item Concentration [mg/L]
NOEC		1000

                                              

The NOEC of the test item Glyceryl Stearate Citrate (Glycerides, C16-18 mono-, di-, and tri-, hydrogenated, citrates, potassium salts) is1000mg/L. The test item is not toxic at the limit concentration of1000mg/L to activated sludge of a municipal sewage treatment plant.

Justification for read-across: The result for CAS 91744-38-6 (Glycerides, C16 -18 mono-, di- and tri-, hydrogenated, citrates, potassium salts) is used in a read-across approach for the registration of CAS 91744-39-7 (Glycerides, C16 -18 mono-, di- and tri-, hydrogenated, citrates, sodium salts). The alkalimetal atoms are not expected to result in differences in toxicological profile and/or environmental fate and behaviour between the two substances.

Besides, water solubility of CAS 91744-38-6 is somewhat higher than water solubility of CAS 91744-39-7. Thus, potential exposure of aquatic organisms to CAS 91744-38-6 – in comparison to CAS 91744-39-7 – is higher when released to the environment and read-across from CAS 91744-38-6 to CAS 91744-39-7 is adequately protective.

For details please refer to the read-across report.