N-methylformamide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions (exposure concentrations in the test and the stability of the test substance were not confirmed by analysis).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412 part 15

Principles of method if other than guideline:

Method: other: DIN 38412, Part 15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

N-methylformamide

EC Number:

204-624-6

EC Name:

N-methylformamide

Cas Number:

123-39-7

Molecular formula:

C2H5NO

IUPAC Name:

N-methylformamide

Details on test material:

- Name of test material (as cited in study report): Monomethylformamide
- Analytical purity: 99.7%

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: golden orfe
- Source: Fish farm P. Eggers, 2354 Hohenwestedt, FRG
- Length at study initiation: 5.3 cm (range: 4.7 - 5.7)
- Weight at study initiation: 3.7 g ( range: 2.1 - 4.9 cm)
- Feeding during test: no food


ACCLIMATION
- Acclimation period: about 7 weeks
- Acclimation conditions: same as test
- Type and amount of food: growing feed F/B 50; SSNIFF Spezialdiaeten GmbH
- Feeding frequency: daily, ad libitum
- Health during acclimation (any mortality observed): during the last 2 weeks 0.04% mortality
- Medical treatment: twice with 0.05 mg/L malachite green chloride; once with 10 mg/L tetracycline hydrochloride

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/L

Test temperature:

20 ±1 °C

Dissolved oxygen:

> 60% of max. saturation

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: all glass aquarium (30 x 22 x 24 cm)
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 3.7 g fish/L test water


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412 part 11
- Alkalinity: 5.5 mmol/L
- Ca/Mg ratio: 4:1
- Na/K ions: 10:1

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

10 000 mg/L

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Any other information on results incl. tables

Sublethal observations / clinical signs:

Effects:

- no observable effect concentration (NOEC; 96 h):  10000 mg/L 

- maximum concentration causing no mortality:  10000 mg/L 

- minimum concentration causing 100 % mortality:  >10000 mg/L (effects related to nominal concentrations) 

 

Symptoms:

- no abnormalities