Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report date:
1987

Materials and methods

Principles of method if other than guideline:
Method: other: DIN 38412, Part 8
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
N-methylformamide
EC Number:
204-624-6
EC Name:
N-methylformamide
Cas Number:
123-39-7
Molecular formula:
C2H5NO
IUPAC Name:
N-methylformamide

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Strain: DSM 50026
- Pretreatment: preincubation 7 ±1 hours

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
17 h

Test conditions

Test temperature:
24 ±1 °C
Nominal and measured concentrations:
Test concentrations (nominal): 312.5, 625, 1250, 2500, 5000 and 10000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 300 mL Erlenmeyer tubes filled with 100 mL test solution
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AK medium according to DIN 38412 part 8

Results and discussion

Effect concentrations
Duration:
17 h
Dose descriptor:
EC10
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Any other information on results incl. tables

pH ranged after 17 h incubation in the different concentrations between 3.7 and 4.8; however at test start without the test organisms the pH ranged between 7.12 and 7.22.

Applicant's summary and conclusion

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