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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions (exposure concentrations in the test and the stability of the test substance were not confirmed by analysis)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Directive 79/831/EEC, Annex V, Part C
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
N-methylformamide
EC Number:
204-624-6
EC Name:
N-methylformamide
Cas Number:
123-39-7
Molecular formula:
C2H5NO
IUPAC Name:
N-methylformamide
Details on test material:
- Name of test material (as cited in study report): Monomethylformamide
- Analytical purity: 99.5 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: obtained from Institute National de Recherche Chimique Appliquee, France. Since 1978 maintained in the laboratory of ecology of BASF AG
- Age at study initiation (mean and range, SD): < 24 h
- Feeding during test: no food

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.7 ±0.5 mmol/L
Test temperature:
21.5 °C
pH:
8.0 - 8.3
Dissolved oxygen:
8.4 - 9.2 mg/L
Nominal and measured concentrations:
Test concentrations: 62.5, 125, 250 and 500 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 20 mL glass beakers filled with 10 mL test solution
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic water according to DIN 38412
- Hardness: 2.7 ± 0.5 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: 600 - 700 µS/cm
- Culture medium different from test medium:
- Intervals of water quality measurement:


OTHER TEST CONDITIONS
- Photoperiod: 12 hours darkness and 12 hours light
- Light intensity: 5 - 6 µE/(m2) wavelength 400 - 700 nm

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Effect values after 24 h:

EC0    = 500 mg/L
EC50   > 500 mg/L
EC100  > 500 mg/L

(values related to nominal concentrations)

Applicant's summary and conclusion

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