Vanadium trichloride oxide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: study not GLP but in which all parameters described are comparable to guideline methods for testing acute toxicity with invertebrates.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

Toxicity of Vanadium to Different Freshwater Organisms.

Author:

Beusen J.M. and Neven B.

Year:

1987

Bibliographic source:

Bulletin of Environmental Contamination and Toxicology, 39, 194-201.

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Remarks:

even if data such as hardness, O2 or photoperiod are lacking

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

sodium metavanadate NaVO3

IUPAC Name:

sodium metavanadate NaVO3

Test material form:

not specified

Details on test material:

purity > 98%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

not specified

Details on test solutions:

Dilution water used for stock cultures and tests is synthetic hard water:
composition (mg/L deionized water):
100 mg NaHCO3,
20 mg KHCO3,
180 mg MgSO4.7H2O
200 mg CaCl2.2H2O

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: daphnids
- Source: obtained from a standardized laboratory culture originally obtained from an uncontaminated pond in the vicinity of the laboratory.
- Age at study initiation: less than 24h old
- Feeding: yes, fed daily with green algae (Chlorella pyrenoidosa)
The stock cultures are held in 3 l beakers containing gently bubble-aerated hard water with a temperature of 19 ± 1°C.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

223 mg/l (as CaCO3 + MgSO4)

Test temperature:

19 °c +/- 1°C

pH:

8.2 - 8.4

Dissolved oxygen:

no data

Salinity:

no data

Nominal and measured concentrations:

Not given, but the measured vanadium concentrations corresponded within 10% of the initial amount of the metal added to each test container.

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 mL borosilicate glass beakers
- Aeration: no aeration during the test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4

EFFECT PARAMETERS MEASURED:
- mortality: determined by lack of movement of antennae or post-abdominal claw on gentle prodding
- complete immobilization: determined by unability of swimming for 15 seconds after gentle agitation

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

4 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

element

Basis for effect:

mortality

Remarks on result:

other: mean of five replicates

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.5 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

element

Basis for effect:

mobility

Remarks on result:

other: mean of five replicates

Any other information on results incl. tables

table 1: summary of results of acute toxicity to Daphnia magna of vanadium (concentrations in mg V/L)

Run	Mortality : 48h LC50	Confidence interval	Mobility : 48h EC50	Confidence interval
1	4.8	4.4- 5.3	3.8	Not calculated
2	3.9	3.1- 4.9	2.9	2.3- 3.7
3	4.3	3.5- 5.3	3.9	3.3- 4.6
4	3.9	2.7- 5.6	3.6	3.0- 4.2
5	3.4	2.7- 4.3	3.3	2.6- 4.0

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Executive summary:

Daphnia magna were exposed to 5 measured vanadium concentrations during 48h under static conditions and in a way similar to the guidelines. Mortality and immobilisation were assessed, and differences between these parameter values were very small. The 48h-NOEC for mobility was determined to be 3.5 mg V/L(geometric mean of 5 replicates).