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EC number: 231-780-2 | CAS number: 7727-18-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- April 2013
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP study performed according to the current guideline, but quoted with reliability 2 due to the read across
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Divanadium pentaoxide
- EC Number:
- 215-239-8
- EC Name:
- Divanadium pentaoxide
- Cas Number:
- 1314-62-1
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Description: orange colored powder
Purity: 99.7%
Expiry date: 24 December 2013
Storage conditions: room temperature in the dark
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The concentration and stability of the test item in the test solutions were verified by chemical analysis at 0 and 72 hours.
The test samples were thawed with the aid of sonication. Nitric acid (2 mL) was added to a volume (100 mL) of sample and the samples were ultrasonicated for 15 minutes.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- An amount of test item (1100 mg) was dispersed in 11 liters of culture medium with the aid of propeller stirring at approximately 1500 rpm for 48 hours. After 48 hours the stirring was stopped and any undissolved test item was removed by filtration through a 0.2 µm Sartorius Sartopore filter (first approximate 2 liters discarded in order to pre-condition the filter) to give a 100% v/v saturated solution.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Culture Collection of Algae and Protozoa (CCAP), SAMS Research Services Ltd, Scottish Marine Institute, Oban, Argyll, Scotland.
- Method of cultivation: maintained in the laboratory by the periodic replenishment of culture medium
- Culturing media and conditions: same as test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 24 ± 1°C
- pH:
- 6.9 to 7.8
- Nominal and measured concentrations:
- 0.68, 2.0, 6.8, 21 and 67 mg/L measured concentrations at 0 hours
99% to 102% of the 0-Hour measured test concentrations were obtained at 72 hours - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass conical flasks were used, constantly shaken at approximately 150 rpm for 72 hours
- cells density: Pre-culture conditions gave an algal suspension in log phase growth characterised by a cell density of 3.74 x 10^ 5 cells per mL. Inoculation of 900 mL of test medium with 12.1 mL of this algal suspension gave an initial nominal cell density of 5 x 10^ 3 cells per mL and had no significant dilution effect on the final test concentration.
- No. of vessels per concentration (replicates): Six flasks each containing 100 mL of solution were used for the control and three flasks each containing 100 mL were used for each treatment group.
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Photoperiod: continuous illumination (intensity approximately 7000 lux)
TEST CONCENTRATIONS
- Range finding study: The results showed no effect on growth at the test concentrations of 0.10 and 1.0% v/v saturated solution. However, growth was observed to be reduced at 10 and 100% v/v saturated solution.
- Test concentrations: 1.0, 3.2, 10, 32 and 100% v/v saturated solution were selected for the definitive test - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 14 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 7.8 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Remarks:
- vanadium
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.8 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 17 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Inhibition of growth rate:
ErC10 (0- 72 h): 14 mg/L
ErC20 (0- 72 h): 15 mg/L
ErC50 (0- 72 h): 17 mg/L
Inhibition of yield:
EyC10 (0- 72 h): 15 mg/L
EyC20 (0- 72 h): 15 mg/L
EyC50 (0- 72 h): 17 mg/L - Reported statistics and error estimates:
- Statistical analysis of the growth rate data was carried out for the control, 0.68, 2.0, 6.8 and 21 mg/L test groups using one way analysis of variance incorporating Bartlett's test for homogeneity of variance (Sokal and Rohlf 1981) and Dunnett's multiple comparison procedure for comparing several treatments with a control (Dunnett 1955). There were no statistically significant differences between the control, 0.68, 2.0 and 6.8 mg/L test concentrations (P≥0.05), however the 21 mg/L test concentration was significantly different (P<0.05) and, therefore the "No Observed Effect Concentration" (NOEC) based on growth rate was 6.8 mg/L.
Correspondingly the "Lowest Observed Effect Concentration" (LOEC) based on growth rate was 21 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- see below in the conclusion field
- Conclusions:
- The following data show that the cell concentration of the control cultures increased by a factor of 96 after 72 hours. This increase was in line with the OECD Guideline that states the enhancement must be at least by a factor of 16 after 72 hours.
Mean cell density of control at 0 hours: 4.54 x 103 cells per mL
Mean cell density of control at 72 hours: 4.35 x 105 cells per mL
The mean coefficient of variation for section by section specific growth rate for the control cultures was 28% and hence satisfied the validation criterion given in the OECD Guideline which states the mean must not exceed 35%.
The coefficient of variation for average specific growth rate for the control cultures over the test period (0 – 72 h) was 2% and hence satisfied the validation criterion given in the OECD Guideline which states that this must not exceed 7%. - Executive summary:
A study was performed to assess the effect of V2O5 on the growth of the green alga Pseudokirchneriella subcapitata. The method followed that described in the OECD Guidelines for Testing of Chemicals (2006) No 201, and the test was performed under GLP procedures.
Chemical analysis of the 1.0, 3.2, 10, 32 and 100% v/v saturated solution test preparations at 0 hours showed measured test concentrations of 0.68, 2.0, 6.8, 21 and 67 mg/L respectively were obtained. Measured concentrations in the range of 99% to 102% of the 0-Hour measured test concentrations were obtained at 72 hours and hence it was considered appropriate to calculate the results based on the 0-Hour measured test concentrations only.
Exposure of Pseudokirchneriella subcapitata to V2O5 gave the following results for inhibition of growth rate:
ErC10 (0 - 72 h): 14 mg/L
ErC50 = 17 mg/L
No Observed Effect Concentration (NOEC) = 6.8 mg/L
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