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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: pre-GLP and pre-guideline study. Missing particle size and animal mortality statistics.

Data source

Reference
Reference Type:
publication
Title:
The Toxicity of Allyl Alcohol. I. Acute and Chronic Toxicity
Author:
Dunlap MK, Kodama JK, Wellington JS, Anderson HH, Hine CH
Year:
1958
Bibliographic source:
AMA Archives of Industrial Health, 18: 303-311

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Single Vapor Exposure: Six male rats were exposed for one, four or eight hours to concentrations ranging from 40 to 23000 ppm. The nominal concentrations were calculated by the standard gas concentration formula of Jacobs and were also checked by chemical analysis. Glass bottles of 1 liter capacity containing distilled water were connected with the sampling port of the chamber, and the vapour drawn through the water by suction. To this aqueous sample was added 0.01 N bromine in acetic acid, in the presence of mercuric acetate catalyst. The excess bromine was reduced by iodide and the iodine titrated with 0.01 N thiosulfate according to the method of Reid and Beddard.
GLP compliance:
no
Test type:
acute toxic class method
Limit test:
no

Test material

Constituent 1
Reference substance name:
Allyl alcohol
EC Number:
203-470-7
EC Name:
Allyl alcohol
Cas Number:
107-18-6
IUPAC Name:
prop-2-en-1-ol
Details on test material:
- Name of test material (as cited in study report): Allyl alcohol

Test animals

Species:
rat
Strain:
Long-Evans
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: no data
- Age at study initiation: no data
- Weight at study initiation: 100 - 200 g
- Fasting period before study: no data
- Housing: in groups of five or six
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: no data

ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): no data

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
air
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: no data
- Exposure chamber volume: 19.5 liters in capacity
- Method of holding animals in test chamber:
- Source and rate of air: air flow from 8.6 to 12.9 L/min, depending on the concentration of allyl alcohol desired
- Method of conditioning air: no data
- System of generating particulates/aerosols: a modified version of the motor-driven syringe assembly described by Carpenter and his associates delivered allyl alcohol from 10 mL Luer-Lok syringe into the chamber through an evaporation through which air was forced at a uniform rate.
- Method of particle size determination: no data
- Treatment of exhaust air: no data
- Temperature, humidity, pressure in air chamber: no data

TEST ATMOSPHERE
- Brief description of analytical method used: no data
- Samples taken from breathing zone: yes

TEST ATMOSPHERE (if not tabulated)
- Particle size distribution: no data
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): no data
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
1 - 8 h
Concentrations:
from 40 to 2300 ppm
No. of animals per sex per dose:
6 rats
Control animals:
no
Details on study design:
- Duration of observation period following administration: at least 10 days after the exposure
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: no data
- Other examinations performed: clinical signs, histopathology
Statistics:
no data

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LC50
Effect level:
795 ppm
Based on:
test mat.
95% CL:
218 - 978
Exp. duration:
1 h
Remarks on result:
other: included 25 % loss of the test item
Sex:
male
Dose descriptor:
LC50
Effect level:
124 ppm
Based on:
test mat.
95% CL:
95 - 169
Exp. duration:
4 h
Remarks on result:
other: included 25 % loss of the test item
Sex:
male
Dose descriptor:
LC50
Effect level:
57 ppm
Based on:
test mat.
95% CL:
50 - 65
Exp. duration:
8 h
Remarks on result:
other: included 25 % loss of the test item
Mortality:
no data
Clinical signs:
other: Apearance of anxiety, lacrimation, tremors. Coma occasionally preceded the death and diarrhea before death.
Body weight:
no data
Gross pathology:
edema and congestion of the lungs (confirmed microscopically), visceral congestion, discolored livers, some with necrotic areas, swollen and discolored kidneys.

Applicant's summary and conclusion

Interpretation of results:
Toxicity Category I
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The LC50 of Allyl alcohol to rats was 124 ppm or 0.297 mg/L.
Executive summary:

In the acute inhalation experiments, six male rats were exposed for one four or eight hours to concentrations ranging from 40 to 2300 ppm. The exposure to the test item was accompanied by an appearance of anxiety, lacrimation and tremors. Diarrhea occured before death and coma occasionally preceded the death. The common gross findings in rats that died were edema and congestion of the lungs (confirmed microscopically), visceral congestion and discolored livers, some with necrotic areas. The kidneys were swollen and discolored. The LC50 of 1, 4 and 8 hours were 795, 124 and 57 ppm respectively (included 25 % loss of allyl alcohol, as stated in the chemical analysis).