N'-(3-aminopropyl)-N,N-dimethylpropane-1,3-diamine

Repeated dose toxicity: inhalation

Administrative data

Endpoint:

short-term repeated dose toxicity: inhalation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

publication

Title:

The subacute inhalation toxicity of 109 industrial chemicals

Author:

Gage JC

Year:

1970

Bibliographic source:

Brit. J. industr. Med., 27, 1-18

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Rats were exposed 6 hours/day for 15 exposures.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-iminodi(ethylamine)

EC Number:

203-865-4

EC Name:

2,2'-iminodi(ethylamine)

Cas Number:

111-40-0

IUPAC Name:

N-(2-aminoethyl)ethane-1,2-diamine

Details on test material:

The sample investigated for inhalation toxicity over this period were submitted by the manufacturing divisions of ICI Ltd.

Test animals

Species:

rat

Strain:

other: Alderley Park specific-pathogen-free

Details on test animals or test system and environmental conditions:

Average weight 200 grams

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

clean air

Remarks on MMAD:

MMAD / GSD: Not applicable

Details on inhalation exposure:

Design of exposure chambers
In all of these experiments the animals have been exposed to dynamic atmospheres, that is, to atmospheres continuously generated and passed through the exposure chamber. The design of exposure chamber has varied with the number of animals involved and with the nature of the substance under investigation. For groups of four or fewer rats a glass desiccator, containing wire mesh partitions to separate the animals, was used. Larger numbers, up to eight rats, were exposed in the chamber described elsewhere (Gage, 1959); usually the inner Perspex chamber of that design was replaced by a glass cylinder, 30 cm diameter and 25 cm high. For atmospheres containing particulate matter a chamber with a more pyramidal top (Gage, 1968) was used.

Generation of the test atmospheres
The air used foi the atmospheres was filtered, dried to a relative humidity of less than 10%, and supplied at a line pressure of 1 atm (1.013 x 10 E5 Nm-2). A nearly saturated vapour obtained by passing air through a liquid contained in a bubbler with a sintered glass air-distributor disc. The volume of the liquid was usually 10-20 ml and, if the size of the sample available permitted, it was replaced daily. Unless otherwise stated, the bubbler was maintained in a water-bath at room temperature, about 20°C.

Analytical verification of doses or concentrations:

yes

Details on analytical verification of doses or concentrations:

The nearly saturated concentration was estimated by weighing the sample before and after the day's run, and telating the weight loss to the volume of air passing. This concentration, expressed in milligrammes per litre, was converted to parts per million on the assumption that the sample was pure. Both of these estimates are only approximate.

Duration of treatment / exposure:

3 weeks

Frequency of treatment:

6-hour/day, 5 days/week

Doses / concentrations

Remarks:

Doses / Concentrations:
550 mg/m3 (130 ppm)
Basis:
analytical conc.

No. of animals per sex per dose:

2

Control animals:

not specified

Details on study design:

- Dose selection rationale: In the initial experiments the concentrations were selected to produce, if possible, acute effects after short exposures.

If at any stage effects were observed which could be attiributed to the exposure, the experiment was repeated with progressively lower concentrations until a concentration was reached which was without effects on the animals. At intervals of about two months, batches of control rats were maintained in a chamber for the exposure period, in order to check the characteristics of the colony.

Examinations

Observations and examinations performed and frequency:

The rats were weighed each morning, and their conditions and behaviour were recorded throughout the exposure period. Urine was collected overnight after the last exposure day for biochemical tests. The animals were left overnight with food and drink. On the following day the rats were anaesthetized with halothane and partially exsanguinated by heart puncture for haematological tests.

Sacrifice and pathology:

GROSS PATHOLOGY: Yes
HISTOPATHOLOGY: Yes

After a gross examination of the organs, the lungs were inflated with formol-saline and immersed in the same fixative. The following organs were also taken for microscopical examination after fixation in formol-corrosive: lungs, liver, kidneys, spleen, and adrenals; and occasionally heart, jejunum, ileum, and thymus.

Results and discussion

Results of examinations

Clinical signs:

no effects observed

Mortality:

no mortality observed

Body weight and weight changes:

not specified

Food consumption and compound intake (if feeding study):

not specified

Food efficiency:

not examined

Water consumption and compound intake (if drinking water study):

not examined

Ophthalmological findings:

not examined

Haematological findings:

no effects observed

Clinical biochemistry findings:

not specified

Urinalysis findings:

no effects observed

Behaviour (functional findings):

not examined

Organ weight findings including organ / body weight ratios:

no effects observed

Gross pathological findings:

no effects observed

Histopathological findings: non-neoplastic:

no effects observed

Histopathological findings: neoplastic:

not examined

Details on results:

DETA at 15 X 6-hour exposures produced no toxic signs (hair coarsened). The autopsy and all organs were normal.

Effect levels

Dose descriptor:

NOAEC

Effect level:

550 mg/m³ air (analytical)

Sex:

male/female

Basis for effect level:

other: no toxic signs (hair coarsened): autopsy, organs normal

Target system / organ toxicity

Critical effects observed:

not specified

Any other information on results incl. tables

The effects of inhaled DETA were examined in a subacute study. Groups of rats were exposed to an essentially saturated vapor of DETA for 6 hrs/day for 15 exposures. There were no effects based on urinalysis or hematological parameters and gross or histopathological examinations.

Applicant's summary and conclusion

Executive summary:

ln a subacute inhalation study, 2 male and 2 female rats (Alderley Park, SPF, 200 g) inhaled diethylenetriamine for 6 hours/ day, 5 days/week for 15 days. The diethylenetriamine-containing atmosphere was produced by passing air through about 10 to 20 ml of the product (which was renewed every day) in a glass inhalation chamber. The calculated concentration of the substance in the chamber was 0.55 mg/1 (130 ppm). The behaviour of the rats and their weight was recorded every day. The rats had ruffled fur but no other signs of intoxication were observed during the entire study. At the end of the study, the animals were killed and post-mortems carried out. The lungs, liver, kidneys, spleen and adrenal glands were examined histologically, but no effects on the organs were found.