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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Version / remarks:
(1984)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2-difluoro-benzo[1,3]dioxole-4-carbaldehyde
EC Number:
700-476-6
Cas Number:
119895-68-0
Molecular formula:
C8H4F2O3
IUPAC Name:
2,2-difluoro-benzo[1,3]dioxole-4-carbaldehyde

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Composite samples (about 150 ml Per concentration and control) of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate Center of the test vessels. They were taken immediately before exposure and after 72 hours exposure and kept at - 18OC to - 22OC until analysis.

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
24°C +/- 1°C
pH:
0h => pH 7.6
72h => pH 8 - 9.9
Nominal and measured concentrations:
nominal measured 0h measured 72h
blank <0.01 mg/ml <0.01 mg/ml
1.23 mg/l 0.88 mg/ml <0.01 mg/l
3.7 mg/l 3.11 mg/ml <0.01 mg/l
11 mg/l 10.2 mg/ml 0.13 mg/l
33 mg/l 33.5 mg/ml 8.7 mg/l
100 mg/l 106.6 mg/ml 70.9 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 ml Erlenmey flasks stoppered with aluminium caps, on Lab-shaker, 50 ml test solution per flask
- Initial cells density: 10100 cells/ml

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Photoperiod: continuous illumination cold white fluoresent light, 128 µE/m2 sec +/- 15% (approx. 8000 lux)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Ce11 densities were measured at 24, 48 and 72 hours exposure on a "TOA" cell Counter.

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.28 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
< 0.13 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
biomass

Applicant's summary and conclusion

Validity criteria fulfilled:
yes

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