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EC number: 221-486-2 | CAS number: 3115-49-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 23, 1986 - February 13, 1986
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-compliant guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- (1981)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- GLP compliance:
- yes
- Remarks:
- Research & Consulting Company AG, CH 4452 Itingen / Switzerland
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- (4-nonylphenoxy)acetic acid
- EC Number:
- 221-486-2
- EC Name:
- (4-nonylphenoxy)acetic acid
- Cas Number:
- 3115-49-9
- Molecular formula:
- C17H26O3
- IUPAC Name:
- 2-(4-nonylphenoxy)acetic acid
- Details on test material:
- - Physical state: oily liquid
- Analytical purity: commercial grade
- Stability of test article dilution: stable for at least 2 hours
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Kleintierfarm Madoerin AG, CH 4414 Fuellinsdorf / Switzerland
- Age at study initiation: 10 to 12 weeks
- Weight at study initiation: Males: 236 - 291 g, Females: 191 - 213 g
- Fasting period before study: 12 to 13 hours
- Housing: Groups of five in Makrolon type-3 cages with standard softwood bedding ("Lignocel", Schill AG, 4132 Muttenz, Switzerland).
- Diet: Pelleted standard Kliba 343, Batch 36/85 rat maintenance diet ("Kliba", Klingentalmuehle AG, 4303 Kaiseraugst, Switzerland) available ad libitum
- Water: Community tap water from Itingen, available ad libitum
- Acclimation period: 1 week
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3
- Humidity (%): 40 - 70
- Air changes (per hr): 10-15
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- polyethylene glycol
- Remarks:
- PEG 400
- Details on oral exposure:
- VEHICLE
- Amount of vehicle (if gavage): 10 ml/kg bw (group 1 and 2), 20 ml/kg (group 3 and 4)
MAXIMUM DOSE VOLUME APPLIED: 20 ml/kg bw
DOSAGE PREPARATION (if unusual):
The test article was placed into a glass beaker on a tared Mettler PK 4800 balance, and the vehicle was added. A weight by volume dilution was prepared using a homogenizer (Ultra-Turrax, Janke and Kunkel, Staufen, FRG). Homogeneity of the test article in the vehicle was maintained during treatment using a magnetic stirrer (Auer-Bittmann, Switzerland). The preparation was made immediately prior to dosing. - Doses:
- 1000, 2000, 3000, 5000 mg/kg bodyweight
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 15 days
- Frequency of observations: Four times during test day 1, and daily during days 2-15.
- Frequency of and weighing: Test days 1 (pre-administration) , 8 and 15.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, eye observations - Statistics:
- The LOGIT-Model (COX, Analysis of Binary Data, London 1977) was applied to estimate the LD50 value. Additionally, the 90, 95 and 99 % confidence intervals for the LD50 for each sex and the slope of the concentration response line were estimated.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 1 674 mg/kg bw
- 95% CL:
- 1 264 - 2 145
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 1 790 mg/kg bw
- 95% CL:
- 1 191 - 2 511
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 1 554 mg/kg bw
- 95% CL:
- 501.9 - 2 663
- Mortality:
- At 1000 mg/kg bw 1 female died (10%)
At 2000 mg/kg bw 3 males and 3 females died (60%)
At 3000 and 5000 mg/bw all animals died (100%) - Clinical signs:
- other: In all treated groups sedation, rales, dyspnea, ataxia, ventral body position (females), latero-abdominal position (female), curved body position and ruffled fur were observed. At 3000 mg/kg chromodaccryorrhea (females) and muscle twitching was observed i
- Gross pathology:
- At necropsy, the following observations were reported in the deceased animals:
-1000 mg/kg: mottled lungs with foam excretion, reddened stomach with whitish content
-2000 mg/kg: mottled lungs with foam excretion, mottled liver, meteorism in stomach/intestines, totally filled with yellowish contents, reddened intestines, filled with whitish or yellowish contents, severely filled urinary bladder and partly yellowish discolored adipose tissue
-3000 mg/kg: mottled lungs with foam excretion, mucous membrane partly reddened, reddened stomach filled with hard or reddish contents, reddened intestine with reddish, whitish, orange or yellow-white content, totally filled urinary bladder, enlarged adrenals (one animal) and partly cannibalism
-5000 mg/kg: mottled lungs with foam excretion, mucous membrane partly reddened, reddened stomach/intestine filled severely yellow-red content, reddened intestines with orange-red content, totally filled urinary bladder.
Applicant's summary and conclusion
- Conclusions:
- The acute oral LD50 of the test item in rats observed over a period of 14 days is 1674 mg/kg bodyweight.
- Executive summary:
In a oral acute toxicity study performed according to OECD guideline 401 and in compliance with GLP, the test article was administered to groups of 5 male and 5 female Wistar rats by gavage at dose levels of 1000, 2000, 3000 and 5000 mg/kg bodyweight. All animals of the two high dose groups died within 6 days. In the low dose group one female died and in the intermediate dose group (2000 mg/kg) 60% of all animals died. The LD50 was set by calculation with the LOGIT-Model for female/male rats at 1674 mg/kg bodyweight. Based on the result of this study, the test article is considered to be moderately toxic after single ingestion.
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