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Administrative data

Key value for chemical safety assessment

Additional information

1,4 -Butynediol was not genotoxic with or without metabolic activation in an Ames test using S. typhimurium strains TA98, TA100, TA1535, and 1537 and test concentrations ranging from 20 - 5000 ug/plate (BASF, 1981). In an in vitro chromosome aberration test with chinese hamster lung fibroblasts, it was determined that 1,4 -Butynediol did not induce chromosomal aberrations and was therefore considered to be non-mutagenic (BG Chemie, 1989). In an in vivo micronucleus test on mouse bone marrow cells, 1,4 -Butynediol did not induce mutagenicity at doses of 17.5, 35, or 70 mg/kg in males and females (BASF, 1989).

Short description of key information:

Negative results for Ames test.

Negative results for in vitro chromosome aberration assay.

Negative results for in vivo micronucleus test.

Endpoint Conclusion: No adverse effect observed (negative)

Justification for classification or non-classification

1,4 -Butynediol (B3D) should not be classified as a mutagen under either the EU DSD classification criteria (Directive 67/548/EEC) or the EU CLP classification criteria (Regulation (EC) 1272/2008) on the basis that B3D was not genotoxic in the Ames test (BASF, 1981), did not induce chromosomal aberrations in an in vitro chromosome aberration test with Chinese hamster lung fibroblasts (BG Chemie, 1989), and did not induce mutagenicity in an in vivo micronucleus test on mouse bone marrow cells (BASF, 1989).