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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The test substance was found to be not readily biodegradable under the test conditions.

Key value for chemical safety assessment

Additional information

The ready biodegradability of the test substance was conducted according to the OECD guideline 301B: CO2 evolution (modified Sturm test). The biodegradation of the test substance was followed by exposing it to microorganisms from the secondary effluent of a domestic waste water treatment plant. As a reference compound (procedure control), Aniline was tested simultaneously under the same conditions. In the toxicity control, containing both test substance and the reference compound Aniline, no inhibitory effect on the microorganisms was observed. In the procedure controls, the reference compound Aniline was readily biodegraded by 94.4% in 28 days. At the end of the test, the test substance biodegraded by an average of 20-33 %. Therefore, the test substance was found to be not readily biodegradable under the test conditions. The mean biodegradation was 26.5%.

The identification of the degradation products of the substance was conducted with Catalogic v5.12.1, CATALOGIC 301C v.10.14 in compliance with Annex XI, paragraph 1.3 of Regulation (EC) No 1907/2006. The substance is in the applicability domain of two of the three submodels but 27.8% fragments are unknown to the model. However, the results support the experimental data on biodegradation and the simulator of metabolism successfully simulated the mineralization (QPRF, iii. Metabolic domain). Overall, the results of the QSAR prediction and the identification of the degradation products can be regarded as reliable.

These QSAR calculations revealed a degradation of 11% after 28d and 17 metabolites were identified. This can be regarded as clear worst case as the model lists the complete degradation map which also contains metabolites at very low quantities as well as metabolites that are subject to further degradation. 12 metabolites were estimated to have a quantity of >= 0.1% mol/mol parent and none of the identified metabolites fulfils the screening criterion for bioaccumulative or very bioaccumulative of logKow >= 4.5. Therefore, the metabolites are not further assessed. Based on the available experimental and modelled data it is assumed as worst case that the substance is persistent in the environment with half lives in fresh water >40d. It can be assumed that some of the relevant degradation products (quantity >0.1% mol/mol parent) are not readily biodegradable and fulfill the criteria for persistence according to REACH Annex XIII as worst case.