Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 209-980-6 | CAS number: 600-00-0
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Qualifier:
- according to guideline
- Guideline:
- other: ICH tripartite harmonised guideline on genotoxicity : specific aspects of regulatory tests
- Qualifier:
- according to guideline
- Guideline:
- other: Japonese MHW
- Qualifier:
- according to guideline
- Guideline:
- other: EPA OPPTS-42193 Mammalian Erythrocyte Micronucleus Test
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- Ethyl 2-bromo-2-methylpropionate
- EC Number:
- 209-980-6
- EC Name:
- Ethyl 2-bromo-2-methylpropionate
- Cas Number:
- 600-00-0
- Molecular formula:
- C6H11BrO2
- IUPAC Name:
- ethyl 2-bromo-2-methylpropionate
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- male/female
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- Corn oil
- Duration of treatment / exposure:
- Once daily
- Frequency of treatment:
- Two consecutive days
- Post exposure period:
- 2 days
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
500, 750, 900, 1000, 2000 mg/kg
Basis:
nominal conc.
Range-finder experiment
- Remarks:
- Doses / Concentrations:
225, 450, 900 mg/kg : males ; 500, 1000, 2000 mg/kg : femals
Basis:
nominal conc.
main study experiment 1
- Remarks:
- Doses / Concentrations:
450 mg/kg
Basis:
nominal conc.
main study experiment 2
- No. of animals per sex per dose:
- 3 males / 3 females per dose : range-finder experiment
6 males / 6 femals per dose : main study experiment 1
7 males : main study experiment 2 - Control animals:
- yes
- Positive control(s):
- Cyclophosphamide (CPA) used as positive control
Examinations
- Tissues and cell types examined:
- Polychromatic / normochromatic erythrocytes
Results and discussion
Test resultsopen allclose all
- Sex:
- male/female
- Genotoxicity:
- positive
- Remarks:
- 450 & 900 mg/kg/day
- Toxicity:
- no effects
- Vehicle controls validity:
- not valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Sex:
- male
- Genotoxicity:
- positive
- Remarks:
- 450 mg/kg/day
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): positive
It is concluded that Ethyl 2-bromoisobutyrate induced micronuclei in the polychromatic erythrocytes of the bone marrow of mice.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
