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Ecotoxicological information

Short-term toxicity to fish

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Link to relevant study record(s)

Description of key information

The substance was harmful to Danio rerio when tested according to OECD Guidelines for Testing of Chemicals (July 1992) No 203. The LC50 observed after 96 hours was 13 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
13 mg/L

Additional information

One acute toxicity study was performed to assess the acute toxicity of eugenol to the freshwater fish species Danio rerio (LAUS GmbH, 2013). The study was conducted under GLP and according to OECD Guideline 203. The study was conducted in a closed semi-static system, and the test substance was replenished every 24 hours on the basis that eugenol is volatile. Fish were exposed to the substance at a range of concentrations from 10 to 100 mg/L in freshwater. The test was performed with 7 fish per vessel and concentration. For each concentration, the percentage of mortality at 24, 48, 72, and 96 hours was recorded. The following OECD 203 validity criteria were met: mortality in the control did not exceed 10% at the end of the test; the dissolved oxygen concentration did not drop below 60% throughout the test; constant conditions (temperature and pH) were within specified deviations; and the concentration of the substance was at least 80% of the nominal concentration throughout the test. Under the conditions of the test, the 96 hour LC50 was 13 mg/L, and therefore the substance is considered harmful to Danio rerio.