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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The substance was toxic to Daphnia magna when tested according to OECD Guidelines for Testing of Chemicals No 202. The EC50 observed after 48 hours was 1.13 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1.13 mg/L

Additional information

One acute toxicity study was performed to assess the acute toxicity of eugenol to Daphnia magna (Bayer, 1999). The study was conducted under GLP and according to OECD Guideline 202. The study was conducted in a static system. Daphnia were exposed to the substance at a range of concentrations from 0.2 to 6.4 mg/L in freshwater. For each concentration, the percentage of immobility at 24 and 48 hours was recorded. Based on data obtained with Bayer (1999) study, CEHTRA (2013) determined that the estimated EC50 value based on the geometric mean of the EC0 and EC100 is not relevant. The EC50 value was recalculated using standard statistical method to be 48h-EC50 = 1.13 mg/L (95% CL: 0.973 - 1.315 mg/L), based on nominal concentrations. This value should be used for classification and risk assessment purposes.