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EC number: 202-589-1 | CAS number: 97-53-0
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 22 June to 24 June 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP study in compliance with international recognised guidelines, only reference to SOP available for analytical methods used.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- To produce the stock solution (10 mg/L), the test substance was weighted into water, treated for 60 seconds at 8000 rpm. with an ultra-turrax and after-wards stirred for 1 hour on a magnetic stirrer.
Separation of test animals according to size before start of test. - Vehicle:
- no
- Details on test solutions:
- See details on sampling.
Test concentrations [mg/L]: 0.2, 0.4, 0.8, 1.6, 3.2, 6.4 - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna STRAUS, parthenogenetic females
Origin of species: cloned at the Federal Health Office (BGA) in Berlin
Age of test animals : 0 - 24 hours
Keeping water: M4-Medium according to Elendt and BGA (1992) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- not applicable
- Hardness:
- 15.3 [°dH]
- Test temperature:
- 19.3ºC
- pH:
- 8.1
- Dissolved oxygen:
- 8.7 - 9.0 mg/L
- Salinity:
- no data
- Nominal and measured concentrations:
- results by start of test and after 48 hours [mg/L]
nominal concentration test substance [mg/L] 0 hours 48 hours
control <0.03 <0.03
0.2 0.2 0.14
0.4 0.41 0.31
0.8 0.82 0.7
1.6 1.55 1.45
3.2 3.16 2.99
6.4 5.71 5.79 - Details on test conditions:
- Operating conditions : Static system in accordance with above study method.
Photoperiod : 16 hours light / 8 hours dark
Test procedure:
-cylindrical test vessels diameter 4.0 cm, height 6.5 cm
- 20 ml test medium
- not ventilated
Method of administration : stock solution (10 mg/L)
Control : M4-Medium according to Elerndt and BGA (1992)
Water for dilution : M4-Medium according to Elendt and BGA (1992) - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.36 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 3.08 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.05 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: geometric mean: EC0/EC100
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 0.973-1.315 mg/L
- Details on results:
- After 48 hours:
EC 0: 0.36 mg/L
EC 100: 3.08 mg/L
Geometric mean:
EC0/EC100: 1.05 mg/L - Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- The EC0 and EC100 are determined directly from the study, while the EC50 (measured) is calculated as the geometric mean of the EC0 and EC100 values.
The 48h-EC50 (nominal) value was calculated with ToxRat to be 1.13 mg/L (95% CL: 0.973 - 1.315 mg/L). - Validity criteria fulfilled:
- yes
- Conclusions:
- The estimated EC50 value based on the geometric mean of the EC0 and EC100 is not relevant. The EC50 value was recalculated using standard statistical method and determined to be 1.13 mg/L.
- Executive summary:
Short-term toxicity to aquatic invertebrates Daphnia magna was assessed according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test), and EU Method C.2 (Acute Toxicity for Daphnia) and in compliance with GLP. Daphnia were exposed under static conditions to the substance at concentrations of 0.2, 0.4, 0.8, 1.6, 3.2, 6.4 mg/L in freshwater.
The test was performed with 10 daphnia per vessel. For each concentration, the percentage of immobilisation at 24 hours and 48 hours was recorded, and test concentrations were confirmed by HPLC analysis.
After 48 hours EC0 was 0.36 mg/L and EC100 3.08 mg/L (measured values), and the EC50 was 1.13 mg/L (nominal).
Reference
Immobile daphnids |
|||||||
Nominal concentration test substance [mg/l] |
24h | 48h | 24h | 48h | Oxygen [mg/ml] 48h |
pH 48h |
Temperature [°C] 48h |
control |
0 | 0 | 0 % | 0 % | 9 | 8.1 | 19.3 |
0.2 | 0 | 0 | 0 % | 0 % | 9 | 8.1 | 19.3 |
0.4 | 0 | 0 | 0 % | 0 % | 8.8 | 8.1 | 19.3 |
0.8 | 0 | 0 | 0 % | 0 % | 8.8 | 8.1 | 19.3 |
1.6 | 7 | 19 | 35 % | 95 % | 8.7 | 8.1 | 19.3 |
3.2 | 8 | 20 | 40 % | 100 % | 8.8 | 8.1 | 19.3 |
6.4 | 12 | 20 | 60 % | 100 % | 8.8 | 8.1 | 19.3 |
No. Parallel solutions: 2
Test volume: 20 ml
No. daphnids per vessel: 10
The values of the abiotic parameters (oxygen, pH and temperature) were determined from the total volume of the parallel solutions.
Description of key information
OECD guideline 202, GLP, key study, validity 1
48h-EC50 = 1.13 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 1.13 mg/L
Additional information
One acute toxicity study was performed to assess the acute toxicity of eugenol to Daphnia magna (Bayer, 1999). The study was conducted under GLP and according to OECD Guideline 202. The study was conducted in a static system. Daphnia were exposed to the substance at a range of concentrations from 0.2 to 6.4 mg/L in freshwater. For each concentration, the percentage of immobility at 24 and 48 hours was recorded. Based on data obtained with Bayer (1999) study, CEHTRA (2013) determined that the estimated EC50 value based on the geometric mean of the EC0 and EC100 is not relevant. The EC50 value was recalculated using standard statistical method to be 48h-EC50 = 1.13 mg/L (95% CL: 0.973 - 1.315 mg/L), based on nominal concentrations. This value should be used for classification and risk assessment purposes.
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