2-ethoxy-5-(4-methyl-1-piperazinylsulfonyl)benzoic acid

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Between 25 November and 9 December 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: done under OECD method and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EEC methods for the determination of ecotoxicity, Directive 92/69/EEC (OJ No. L383A, 29.12.92), Part C, Method C7, Abiotic degradation: hydrolysis as a function of pH; and the estimation of the soil adsorption coefficient (Koc) by HPLC

Principles of method if other than guideline:

The method is only applicable to water soluble substances

GLP compliance:

yes

Test material

Test material information

Constituent 1

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Intended use: Pharmaceutical intermediate
Appearance: White powder
Storage conditions: Room temperature
Purity: 99.4%
Date received: 2 October 1996

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No Data

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Duration of test (contact time):

28 d

Details on study design:

TEST CONDITIONS
- Test temperature: XX C
- pH: XX
- pH adjusted: yes or no
-Dilution water: HQ deionised water with conductivity of < 10 uS/cm and low carbon content (modfy as needed)

TEST SYSTEM - CHECK thse are the same
- Culturing apparatus: series of glass carboys containing 3 liters of test medium
- Number of culture flasks/concentration: 2 test flasks with test substance and inoculum
- Method used to create aerobic conditions: carbon dioxide free air

CONTROL AND BLANK SYSTEM -check these are the same
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
- Other: 1 system blank, 1 procedure blank

Reference substance

Reference substance:

other: Sodium benzoate

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

27

Sampling time:

28 d

Details on results:

Points of degradation plot (test substance): vv % degradation after 1 d vv % degradation after 2 d vv % degradation after 3 d vv % degradation after 6 d vv % degradation after 8 d vv % degradation after 10 d vv % degradation after 14 d vv % degradation after 16 d vv % degradation after 20 d vv % degradation after 22 d vv % degradation after 24 d vv % degradation after 27 d vv % degradation after 28 d

BOD5 / COD results

Results with reference substance:

Points of degradation plot (reference substance): vv % degradation after 1 d vv % degradation after 2 d vv % degradation after 3 d vv % degradation after 6 d vv % degradation after 8 d vv % degradation after 10 d vv % degradation after 14 d vv % degradation after 16 d vv % degradation after 20 d vv % degradation after 22 d vv % degradation after 24 d vv % degradation after 27 d vv % degradation after 28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other:

Conclusions:

The test material attained vv% degradation after 28 days and therefore cannot (or can) be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No 301B.