Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: According to international guideline(s), GLP-compliant, performed in recognized contract research organization, no restrictions, fully adequate for assessment.
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
;2004
Deviations:
no
Qualifier:
according to guideline
Guideline:
ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
Version / remarks:
;1998
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
A stock solution of 1000 mg/L was prepared in test medium. The stock solution was stirred at 30°C for 2 hours.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Source: in-house culture originating from Daphnia magna Straus collected in Langedam, Birkerød, Denmark; cultured at DHI since 1979
- Age at study initiation: < 24 h
- Feeding during test: not indicated
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
250 ± 25 mg/L as CaCO3
Test temperature:
20 °C ± 0.4 °C
pH:
7.7 - 7.9
Dissolved oxygen:
95 - 99 % of saturation
Nominal and measured concentrations:
nominal concentrations: 10, 20, 50, 100, 200, 500, and 1000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass containers containing 25 mL of test solution
- Renewal rate of test solution (frequency/flow rate): after 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 6
- Biomass loading rate: 5 mL medium per organism

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deinonised water

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: the test was performed in total darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : numbers of immobile animals was recorded 24 hours (before and after changing the test medium) and 48 hours after the start of the study

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.0-2.5
- Range finding study: not reported
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
176 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: upper 95% confidence limit: 193 mg/L; lower limit not calculabe
Key result
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC10
Effect conc.:
146 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: upper 95% confidence limit: 178 mg/L;(lower limit not calculabe
Details on results:
- Immobilised Daphnia: see Table 1
- Mortality of control: none
Results with reference substance (positive control):
- Results with reference substance valid? Yes (range specified in ISO 6341 (0.6-2.1 mg/L)
- EC50/LC50: 24-hour EC50: 1.9 mg/L (95% confidence limits 1.7-2.2 mg/L).
Reported statistics and error estimates:
The EC10 and EC50 values were calculated by Probit Analysis.

Table 1: Cumulative immobilisation

 

Nominal concentration (mg/L)

Cumulative numbers of immobile Daphnia

24 hours

48 hours

total

%

total

%

Control

0

0

0

0

10

0

0

0

0

20

0

0

0

0

50

0

0

0

0

100

0

0

0

0

200

9

45

17

85

500

20*

100

20

100

1000

20**

100

20

100

*: 90% of the test organisms were immobile after 20 min

**: 100% of the test organisms were immobile after 20 min

Validity criteria fulfilled:
yes
Remarks:
In the control, no Daphnia have been immobilised. The dissolved oxygen concentration at the end of the test was ≥3 mg/L (≥60% of the air saturation value at the temperature used).

Description of key information

48-h EC50 > 100 mg/L for Daphnia magna (OECD TG 202, ISO 6341))

Key value for chemical safety assessment

Additional information