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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
11 October 1984 to 19 October 1984
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
comparable to guideline study
Qualifier:
according to guideline
Guideline:
other: ETAD-Method No.103
Deviations:
no
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
no
Specific details on test material used for the study:
Test substance FAT-20306/B
Storage: room temperature
Appearance: solid
Analytical monitoring:
no
Details on sampling:
no data
Vehicle:
no
Details on test solutions:
no data
Test organisms (species):
activated sludge
Details on inoculum:
Test organism: mixed culture of bacteria (sludge of Husman-apparatus)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Hardness:
no data
Test temperature:
20 ±2 °C
pH:
no data
Dissolved oxygen:
not applicable
Salinity:
no data
Nominal and measured concentrations:
nominal: 1, 3.2, 10, 32, 100 mg/l
Details on test conditions:
no data
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
test concentration(mg/l) inhibition (%)
1 1.8
3.2 8.2
10 11.8
32 29.1
100 31.2
Results with reference substance (positive control):
The IC50 (3 h) of the reference substance, 3,5-Dichlorophenol is: 19 mg/l (Criteria: 10-25 mg/l)
Reported statistics and error estimates:
Calculate the respiration rate of each test concentration as a percentage of the mean of the two control respiration rates. Plot the results on a log (concentration)/normal (% inhibition) graph and derive an IC50 value.
Validity criteria fulfilled:
yes
Conclusions:
IC50 (3h) of FAT-20'306/B was >100 mg/L.
Executive summary:

The determination of IC50 study was performed with FAT 20306/B in accordance with the ETAD Method No. 103 corresponding to OECD-Method No.209. The nominal concentrations used were 1, 3.2, 10, 32, 100 mg/L. Based on the test results, the IC50 (3 h) of FAT-20306/B was >100 mg/L.

Description of key information

The determination of IC50 study was performed with FAT 20306/B in accordance with the ETAD Method No. 103 corresponding to OECD-Method No.209. The nominal concentrations used were 1, 3.2, 10, 32, 100 mg/L. Based on the test results, the IC50 (3 h) of FAT-20'306/B was >100 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
100 mg/L

Additional information