C.I. Acid Yellow 246

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11 October 1984 to 19 October 1984

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

other: ETAD-Method No.103

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

no

Specific details on test material used for the study:

Test substance FAT-20306/B
Storage: room temperature
Appearance: solid

Analytical monitoring:

no

Details on sampling:

no data

Vehicle:

no

Details on test solutions:

no data

Test organisms (species):

activated sludge

Details on inoculum:

Test organism: mixed culture of bacteria (sludge of Husman-apparatus)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Hardness:

no data

Test temperature:

20 ±2 °C

pH:

no data

Dissolved oxygen:

not applicable

Salinity:

no data

Nominal and measured concentrations:

nominal: 1, 3.2, 10, 32, 100 mg/l

Details on test conditions:

no data

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

test concentration(mg/l) inhibition (%)
1 1.8
3.2 8.2
10 11.8
32 29.1
100 31.2

Results with reference substance (positive control):

The IC50 (3 h) of the reference substance, 3,5-Dichlorophenol is: 19 mg/l (Criteria: 10-25 mg/l)

Reported statistics and error estimates:

Calculate the respiration rate of each test concentration as a percentage of the mean of the two control respiration rates. Plot the results on a log (concentration)/normal (% inhibition) graph and derive an IC50 value.

Validity criteria fulfilled:

yes

Conclusions:

IC50 (3h) of FAT-20'306/B was >100 mg/L.

Executive summary:

The determination of IC50 study was performed with FAT 20306/B in accordance with the ETAD Method No. 103 corresponding to OECD-Method No.209. The nominal concentrations used were 1, 3.2, 10, 32, 100 mg/L. Based on the test results, the IC50 (3 h) of FAT-20306/B was >100 mg/L.

Description of key information

The determination of IC50 study was performed with FAT 20306/B in accordance with the ETAD Method No. 103 corresponding to OECD-Method No.209. The nominal concentrations used were 1, 3.2, 10, 32, 100 mg/L. Based on the test results, the IC50 (3 h) of FAT-20'306/B was >100 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

100 mg/L