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Specific investigations: other studies

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Administrative data

Type of information:
experimental study
Adequacy of study:
supporting study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference Type:

Materials and methods

Principles of method if other than guideline:
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazorium bromide (MTT) colorimetric assay; the cytotoxic effect of DMA on the cell viability in human salivary gland (HSG) cells (salivary gland carcinoma cell line) and human gingval fibroblasts (HGF) cells was determined.
GLP compliance:
not specified
Type of method:
in vitro

Test material

Details on test material:
2-Propenoic acid, 2-methyl-, dodecyl ester purity not specified, but commercial grade assumed.
Specific details on test material used for the study:
- Supplier: Tokyo Kasei Cem. Co., Tokyo, Japan
- Purity: commercial grade, not further purified

Results and discussion

Details on results:
The cytotoxic effect of DMA on the cell viability of human salivary gland (HSG) cells (salivary gland carcinoma cell line) was CC50: 0.001 mM (cytotoxic concentration for 50 % cell death) and human gingval fibroblasts (HGF) cells was CC50: 0.001 mM (cytotoxic concentration for 50 % cell death). The CC50 values were determined from the dose-response curves.
In the HGF cells the methacrylate-induced increase in Ca2+ elevation required higher monomer concentrations than in HSG cells.
The Ca2+ mobilisation activity stimulated by DMA was 0.1 mM (threshold concentration for peak appearance) for HSG cells. The DMA-stimulated peak, which was present at 0.1 and 0.5 mM was absent at concentrations of 1 mM and higher.

Applicant's summary and conclusion

The mechanism of interaction of the investigated monomers with cell membranes remains unkonwn with respect to cytotoxicity and calcium release.
Executive summary:

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