Carboxylic acids, di-, C4-6

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

48 h

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

46 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: immobilization

Details on results:

As no information is given on analytical measurements, it is assumed that the effect concentrations relate to nominal concentrations.

No datails given on pH.

Validity criteria fulfilled:

yes

Conclusions:

The test gave a 48-h EC50 value of 46 mg/L.

Executive summary:

Assessing the toxicity to aquatic invertebrates, Daphnia magna was exposed to adipic acid for 48 hours. The experiment was conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The test gave a 48-h EC50 value of 46 mg/L.

Description of key information

Describing the toxicity towards aquatic invertebrates, a study conducted by the National Institute of Technology and Evaluation, Japan is taken into account, using adipic acid as test substance. Assessing the toxicity of adipic acid towards aquatic invertebrates an experiment was conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The test gave an 48-h EC50 value of 46 mg/L (MITI, 1997).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

46 mg/L

Additional information

With the invertebrate Daphnia magna one acute test according to the European guideline 79/831/EEC, method C.2 is available. For a test period of 24 hours an EC50 value of 88.4 mg/l was obtained. The same effect concentration was reported after a test period of 48 hours (BASF AG 1988). pH values in the test solutions ranged from 4 (500 mg/l) to 7.2 (31.3 mg/l) and pH related effects on the daphnids cannot be excluded. This study on daphnids was characterized by a concentration-dependant decrease of pH. During the test on Daphnia, the treatments testing dicarboxylic acid's concentrations near the EC50 value presented a pH markedly inferior to the control treatment. At the end of the 48-hour exposure period, the pH in the control treatment was 8.2, whereas it decreased from 7.2 to 5.2 in the range of concentrations (62.5 - 125 mg/L) covering the EC50 value (88.4 mg/L). Furthermore, it should be noticed that the pH value in the control treatment is high compared to the guideline's requirements. Based on this observation, a pH effect cannot be ruled out when considering the toxicity reported in this study.

Describing the toxicity towards aquatic invertebrates, a study conducted by the National Institute of Technology and Evaluation, Japan is taken into account, using adipic acid as test substance. The toxicity of adipic acid towards aquatic invertebrates was assessed in an experiment conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The test gave an 48-h EC50 value of 46 mg/L (MITI, 1997).