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Short-term toxicity to aquatic invertebrates

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Description of key information

The substance was not acutely toxic to Daphnia magna when tested according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test); the substance is predicted to possess a 48-hour EC50 in Daphnia magna that is equal to 308 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
308 mg/L

Additional information

Three studies addressing short-term toxicity to aquatic invertebrates are presented in the dossier. Please refer to the document attached to Section 13 for further discussion of the studies.

Short-term toxicity to aquatic invertebrates.001 – Daphnia – 1998 – key: Acute toxicity to Daphnia magna was assessed according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test), and in compliance with GLP. Daphnia were exposed under static conditions to the substance at concentrations of 52, 86, 144, 240, and 400 mg/L in freshwater. The test was performed with 10 daphnia per vessel and 20 daphnia per concentration. For each concentration, the percentage of immobilisation at 24 hours and 48 hours was recorded, and test concentrations were confirmed by GC analysis. The following validity criteria were met: immobilisation in the control did not exceed 10% at the end of the test; dissolved oxygen concentrations remained above 60% of the air saturation value at the end of the test; pH did not vary by more than one unit throughout the test; and the concentrations of the test substance remained within 80% of the initial concentration throughout the test. The measured concentrations at the end of the test were 48, 68, 136, 234, and 345 mg/L which were used to express the results of the test. After 48 hours immobility in the 136, 234 and 345 mg/L treatment groups was 0, 5 and 75% respectively.   Under the conditions of the test, the 48-hour EC50 was 308 mg/L, and therefore the substance was not acutely toxic to Daphnia magna.

Short-term toxicity to aquatic invertebrates.002 - Daphnia - 1982: Acute toxicity to Daphnia magna was not assessed according to a specific guideline, however; it appears to follow OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) but was not conducted according to GLP (pre-dates GLP). The Daphnia were exposed to the substance under static conditions for 24 hours and the 24-hour EC50 for immobility was determined to be 7060 mg/L. This lower toxicity over a shorter exposure period is in keeping with the result of the key study.

Short-term toxicity to aquatic invertebrates.003 - Daphnia - 1977: Acute toxicity to Daphnia magna was not assessed according to a specific guideline, however; it appears to follow OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) but was not conducted according to GLP (pre-dates GLP). The Daphnia were exposed to the substance under static conditions for 24 hours and the 24-hour EC50 for immobility was determined to be 8890 mg/L. This lower toxicity over a shorter exposure period is in keeping with the result of the key study.