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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The test substances, Fluidized Bed Combustion (FBC) Fly Ash and Fluidized Bed Combustion (FBC) Bottom  Ash, were tested for acute toxicity on fish Poecilia reticulata.
The tests were performed according to method C.1 – Acute toxicity for fish, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
Since the test substances are poorly soluble in dilution water at concentrations needed for the test, a stock saturated solution (nominal concentration 100 mg.L-1) of the test substance in dilution water was prepared for preliminary and limit test.
The studies were performed without the analytical determination of the test substances concentrations. The nominal concentrations of the test substance were used for all evaluations and results. Only concentrations of metals in stock saturated solution were analysed in the limit test.
The range of water solubility of FBC Ashes is 0.67 to 4.619 g/L at the temperature of 20oC. FBC Ashes are moderately soluble to soluble.

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

Fluidized Bed Combustion (FBC) Fly Ash: No mortality was observed in limit test.

Fluidized Bed Combustion (FBC) Bottom Ash: The highest observed mortality was 10 % in the limit test.

Therefore exact values of LC50could not be calculated and the values of LC are given in the form of a range.

The NOEC values are identified by direct observation.

The nominal concentrations of the test substance were used for all evaluations and results.