Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 235-123-0 | CAS number: 12070-12-1
Toxicological information
Acute Toxicity: oral
Currently viewing:
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1998-02-24 to 1999-06-24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Well documented, scientifically sound study that followed OECD Guideline 401 and GLP.
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
- Reference Type:
- publication
- Title:
- SIDS Initial Assessment Report for SIAM 21 for Tungsten Carbide (12070-12-1), Washington DC,18-20 October, 2005
- Author:
- OECD-SIDS
- Year:
- 2 005
- Bibliographic source:
- UNEP Publications
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- yes
- Remarks:
- The lowest humidity recorded was 29%. This was lower than the range of 30 - 70% stated in the protocol. This deviation was not considered to have affected the integrity or validity of the study.
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Tungsten carbide
- EC Number:
- 235-123-0
- EC Name:
- Tungsten carbide
- Cas Number:
- 12070-12-1
- Molecular formula:
- CW
- IUPAC Name:
- tungsten carbide
- Details on test material:
- - Test substance: tungsten carbide, WC HC 240
- Purity: 99.98%
- Physical state: Grey powder
- Storage condition of test material: Room temperature
- Expiration date of the lot/batch: Not advised
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Hsd: Sprague-Dawley(CD))
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Obtained from Harlan U.K. Ltd, Bicester, Oxon, England.
- Age at study initiation: four to seven weeks or age.
- Weight at study initiation: range of 80 to 101 g.
- Fasting period before study: Access to food only was prevented overnight prior to and for approximately 4 hours after dosing.
- Housing: Rats were allocated without conscious bias to cages within the treatment group and housed in groups of five rats of the same sex in metal cages with wire mesh floors.
- Diet (e.g. ad libitum): A standard laboratory rodent diet (Special Diet Services RM1(E) SQC expanded pellet)
- Water: drinking water provided ad libitum
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature: 21 to 22.5 degree C
- Humidity: 9-52%
- Air changes: Air exchange was maintained at 10 to 15 air changes per hour.
- Photoperiod: lighting controlled by means of a time switch to provide 12 hours of artificial light (0700 - 1900 hours).
IN-LIFE DATES: From: 1998-02-24 To: 1998-03-10
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: 1 % w/v aqueous methylcellulose
- Details on oral exposure:
- The test substance was formulated at a concentration of 20 % (w/v) in 1 % (w/v) aqueous methylcellulos which was administered at a volume of 10 mL/kg-bw.
- Doses:
- single oral gavage dose of 2000 mg/kg bw
- No. of animals per sex per dose:
- 5 male and 5 females
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Statistics:
- no data
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Sex:
- male/female
- Dose descriptor:
- other: NOEL
- Effect level:
- > 2 000 mg/kg bw
- Mortality:
- 0/5 for males and females
- Clinical signs:
- Piloerection was observed in all rats within three minutes of dosing. This sign persisted and was accompanied on Day 2 only by ungroomed appearance (characterised by ungroomed coat) in all males. There were no other signs of reaction to treatment and recovery was complete in all animals by Day 3.
- Body weight:
- No changes in body weight gain were seen.
- Gross pathology:
- No macroscopic abnormalities at terminal kill on day 15 were observed.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The acute lethal oral dose to rats of Tungsten Carbide Powder - Pure was demonstrated to be greater than 2000 mg/kg bodyweight.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Route: .live1