Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs.

Toxicity to terrestrial arthropods

Administrative data

Endpoint:

toxicity to non-target arthropods on natural substrate (NTA other than pollinators)

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well-documented, peer-reviewed publication.

Data source

Reference

Reference Type:

publication

Title:

Effects of an Anionic Surfactant, Linear Alkylbenzene Sulfonate, on Survival, Reproduction and Growth of the Soil-Living Collembolan Folsomia Fimetaria

Author:

Holmstrup, M, and Krogh, PH

Year:

1996

Bibliographic source:

Environmental Toxicology and Chemistry, Vol. 15, No. 10, 1745-1748

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Groups of 10 male and 10 female F. fimetaria were exposed to concentrations of LAS ranging from 0 -1000 mg/kg dw soil for 21 days. At the end of the 21 days, the number of F. fimetaria were counted, and the number of surviving adults and juveniles as the result of reproduction were counted.

GLP compliance:

no

Application method:

soil

Test material

Test material information

Constituent 1

Reference substance name:

Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs.

EC Number:

287-494-3

EC Name:

Benzenesulfonic acid, 4-C10-13-sec-alkyl derivs.

Cas Number:

85536-14-7

Molecular formula:

None available - not a single isomer - see remarks

IUPAC Name:

4-(tridecan-3-yl)benzene-1-sulfonic acid

Details on test material:

- Name of test material (as cited in study report): Marlon A 350
- Molecular weight (if other than submission substance): 344 g/mol mean molecular weight
- Analytical purity: 50% active substance
- Composition of test material, percentage of components: average chain length = 11.53

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples were taken 2, 17, and 31 days after the soil was spiked.

Test substrate

Details on preparation and application of test substrate:

LAS was dissolved in water and mixed with 30 g moist soil (25.5 g dry). The mixture was prepared immediately before the experiment.

Test organisms

Test organisms (species):

Folsomia sp.

Animal group:

Collembola (soil-dwelling springtail)

Details on test organisms:

TEST ORGANISM
- Source: laboratory culture
- Age at test initiation (mean and range, SD): 23-26 days
- Weight at test initiation (mean and range, SD):

Study design

Study type:

laboratory study

Total exposure duration:

21 d

Test conditions

Test temperature:

20 degrees C

pH (if soil or dung study):

5.5

Photoperiod and lighting:

12 hrs light/12 hrs dark

Details on test conditions:

TEST SYSTEM
- Amount of soil or substrate: 30 g moist
- No. of organisms per container (treatment): 10 male/10 female
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Speyer, Germany
- Soil texture (if natural soil)
- % sand: 34.8
- % silt: 5.6
- % clay: 5.2
- % gravel: 54.6
- Organic carbon (%): 2.3
- Pretreatment of soil: dried at 80 degrees C
- Storage (condition, duration): 5 degrees C

OTHER TEST CONDITIONS
- Photoperiod: 12/12 hrs

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : survival and reproduction at 21 days of exposure measured using digital image processing

Nominal and measured concentrations:

nominal: 0-1000 mg/kg dry soil

Results and discussion

Effect concentrations

Duration:

21 d

Dose descriptor:

EC10

Effect conc.:

147 mg/kg soil dw

Nominal / measured:

nominal

Basis for effect:

reproduction

Remarks on result:

other: 116-178

Details on results:

Adult survival was unaffected at all concentrations. Reproductive output decreased linearly with increasing concentration, with 430 juveniles produced in control containers, to only 150 juveniles produced at the highest concentration (1000 mg/kg dw soil).

Applicant's summary and conclusion

Conclusions:

The 21-day EC10 for F. fimetaria was 147 mg/kg dw soil based on reproduction.

Executive summary:

Groups of 10 male and 10 female F. fimetaria were exposed to concentrations of LAS ranging from 0 -1000 mg/kg dw soil for 21 days. At the end of the 21 days, the number of F. fimetaria were counted, and the number of surviving adults and juveniles as the result of reproduction were counted. There was no effect on adult survival, however, there was a dose-related decrease in reproduction. The EC10 based on reproduction was 147 mg/kg dw soil.