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Diss Factsheets

Toxicological information

Acute Toxicity: inhalation

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Administrative data

acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Comparable to guideline study with limitations which do not impair the overall conclusion from the data. Sufficiently detailed description of the test procedure in accordance with scientific standards (critical supporting information given, such as species tested, substance identity and dosing procedure).

Data source

Reference Type:
Silicon Carbide Induced Pneumoconiosis: A Microscopic and Biochemical Experimental study
M Petran, A Cocârla, DC Olinici
Bibliographic source:
J Occup Health 1999; 41: 253- 258

Materials and methods

Principles of method if other than guideline:
experimental model by intratracheal instillation, BAL analyses
GLP compliance:
not specified

Test material

Chemical structure
Reference substance name:
Silicon carbide
EC Number:
EC Name:
Silicon carbide
Cas Number:
Molecular formula:

Test animals

guinea pig

Administration / exposure

Route of administration:
other: suspended in physiological solution
Type of inhalation exposure:
other: intratracheal instillation
other: physiological solution
Remarks on duration:
single dose
50 mg standardized SiC suspended in 1 ml physiological solution
No. of animals per sex per dose:
Control animals:
statistical method employed: Student's test

Results and discussion

Effect levels
Dose descriptor:
other: non applicable
Remarks on result:
other: SiC particles do not cause lung fibrosis, and in fact, behave in the lung as do other inert dusts such as latex beads or graphite dust.
Body weight:
Whereas the weight of the control animals showed a slight constant increase, in the SiC treated animals a transient decrease was found after instillation, which subsequently returned to normal.
Other findings:
- Lung weight was significantly higher in the SiC treated group.
- Fibrotic lesions following the formation of cell granulomas were observed in the SiC treated group.
- Lung tissue hydroxyproline was increased in the SiC treated group.

Applicant's summary and conclusion

Executive summary:

The pneumoconiogenic effects of silicon carbide were studied in an experimental model by intratracheal instillation of a dose ol 50 mg silicon carbide, standardized and commercialized by Duke Scientific Corporation, in a group of 30 guinea pigs killed at 40, 70 and 100 days, respectively. Lung samples were obtained and they were fixed, included, sectioned, stained with hematoxylineosin, van Gieson and Masson's trichromic and studied under the optical microscope. The microscopic aspects were compared with those of the controls (10 guinea pigs), intratracheally instilled with 1 ml physiological solution (NaCl 0,9%) at the beginning of the experiment and killed with the last group of guinea pigs at 100 days. The optical microscopic study showed important changes in the pulmonary structure: alveolar wall ruptures, infiltration of alveolar septa with round cells and histiocytes, as well as diffuse interstitial fibrosis which was more obvious 100 days after the instillation. Lung tissue hydroxyproline, as a marker of collagen synthesis, showed a significant increase correlated with the ocurrence of fibrosis. The results of the experimental research support the pneumoconiogenic effect of silicon carbide.