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Short-term toxicity to aquatic invertebrates

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Key value for chemical safety assessment

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A sample of diesel fuel was tested in a static 48 -hour acute daphnid (Daphnia magna) test (OECD 202; KS = 1).

Twenty organisms / dose were exposed to diesel fuel WAFs at nominal concentrations of 0, 10, 22, 46, 100, 220, 460 and 1000 mg/L. The EL50 was 210 mg/L.


Supporting PETROTOX (version 3.06) modelled values are available. The aquatic toxicity was estimated using the PETROTOX computer model, which combines a partitioning model (used to calculate the aqueous concentration of hydrocarbon components as a function of substance loading) with the Target Lipid Model (used to calculate acute and chronic toxicity of non-polar narcotic chemicals). PETROTOX computes toxicity based on the summation of the aqueous-phase concentrations of hydrocarbon block(s) that represent a petroleum substance and membrane-water partition coefficients (KMW) that describe the partitioning of the hydrocarbons between the water and organism. 

The model predicted LL50 of the substance to aquatic invertebrates to be 33.87 mg/L.