2-(diethylamino)ethyl methacrylate

Endpoint summary

Additional information

The test substance tends to split immediately to two components in aqueous solution. Its half-life is about 1 hour, and it almost fully decomposes in 5 to 6 hours. Therefore, in all tests performed, concentration of the test substance in the test solution was not measured. Thus, the test result was calculated in accordance with a target concentration.
 
Short-term toxicity to fish
Acute toxicity Test (96 hours) to fish (Japanese killifish (Oryzias latipes)) was assessed similarly to OECD 203 guideline with 100 mg/L of the test substance (limit test). Under the test conditions the LC50 (96 h) value obtained was greater than 100 mg/L. A prolonged toxicity test to fish was performed similarly to the OECD 204 guideline with Japanese killifish (Oryzias latipes). The test substance (100 mg/L) was applied for different exposure period (14 days) and exposure method (flow-through) than the in the study detailed above. Under the test conditions the LC50 (14 d) value obtained was also here greater than 100 mg/L. This result supports the the LC50 (96 h) of greater than 100 mg/L.

Long-term (prolonged) toxicity to fish
Long-term toxicity to fish (Japanese killifish (Oryzias latipes)) was assessed similarly to OECD 204 guideline. The test substance was applied for 14 days in the following nominal concentrations: 30.9, 55.6, 100 mg/L. Under the test conditions the NOEC value obtained was 100 mg/L.
 
Short- term toxicity to aquatic invertebrates
Daphnia (daphnia magna) acute immobilization test (48 hours) was assessed similarly to OECD 202 guideline with the test substance in the following concentrations: 53, 95, 171, 309, 556, 1000 mg/L. Under the test conditions the EC50 value (48 h, immobilization) obtained was 362 mg/L.
 
Long- term toxicity to aquatic invertebrates
Daphnia reproduction test (21 days) was assessed similarly to OECD guideline 211 with the test substance in the following concentrations: 55.6, 100 mg/L. Under the test conditions the NOEC (reproduction) and the LOEC (reproduction) values obtained were 55.6 mg/L and 100 mg/L, respectively.
 
Toxicity to aquatic alga
Toxicity to aquatic algae was assessed according OECD guideline 201 with the test substance in concentrations of 0.53, 0.95, 1.7, 3.09, 5.56, 10.0 mg/L. Results obtained showed that the growth inhibition concentration by comparison of growth rate for 24 - 72 hours, ErC50 (24 - 72) was 9.84 mg/L, and its 95 % confidence interval was 8.33 - 12.25 mg/L. The NOEC (Velocity method 24-72) was 3.10 mg/L or more. The growth inhibition concentration by comparison of growth rate, ErC50 (24 - 48) was 10.00 mg/L or more and the NOEC (Velocity method 24 - 48) was 10.00 mg/L or more. The growth inhibition concentration by comparison of areas under growth curve, EbC50 (0 - 72) was 3.74 mg/L (95 % Confidence interval: 3.26 mg/L - 4.34 mg/L) and the NOEC (Area method 0-72) was 1.00 mg/L.
The following results were taken into account: EC50 value of 3.74 mg/L and NOEC value of 1.00 mg/L based on biomass, obtained for the whole study exposure period (0-72 hours). On the other hand EC50 value of 9.84 mg/L and NOEC value of 3.10 mg/L based on growth rate, obtained for study exposure period of 24-72 hours (but not 0 -72 hours) are available. When additionally, supposing that minimal change occurred at exposure time 0-24 hours (no data was available for this time point), it can be assumed that the NOEC value arising from these results and relevant for C&L is between more than 1 mg/L to 10 mg/L. ErC50 (24-72 hours) of 9.84 mg/L and NOEC (based on growth rate, 24 -72 hours) of 3.10 were considered for PNEC calculations being the most suitable available parameters/ results.
In conclusion, since the substance is ready biodegradable, these results do not trigger classification and labelling with respect to chronic aquatic toxicity.