Tin sulfide (Sn2S3)

Endpoint summary

Description of key information

Key GLP and guideline compliant studies on a structural analogous read-across substance tin sulfide and tin disulfide are availble for oral, inhalatoin and dermal toxicity testing. Alles studies showed values above limit dose, therefore ditin trisulfide shows no acute toxicity.

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records

Reference

Endpoint:

acute toxicity: oral

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Read-Across Justification_SnS and SnS2 to Sn2S3

Reason / purpose for cross-reference:

read-across source

Limit test:

yes

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 213 mg/kg bw

Based on:

other: calculated for ditin trisulfide

Mortality:

All animals survived the 2000 mg/kg oral dose.

Clinical signs:

other: All animals survived the 2000 mg/kg oral dose, all body weights were normal for both sexes. There were no abnormal physical signs noted during the observation period. Necropsy results were normal.

Gross pathology:

All animals survived the 2000 mg/kg oral dose, all body weights were normal for both sexes. There were no abnormal physical signs noted during the observation period. Necropsy results were normal.

Interpretation of results:

not classified

Remarks:

Criteria used for interpretation of results: EU

Conclusions:

The Acute Toxic Class Determination according to OECD guideline 423 revealed an LD50 is > 2000 mg/kg bw for read-across substance tin sulfide in rats.
Calculated for ditin trisulfide the LD50 value is > 2213 mg/kg bw.

Executive summary:

The Acute Toxic Class Determination according to OECD guideline 423 was used to determine the oral toxicity of read-across substance tin sulfide in rats.

All animals survived the 2000 mg/kg oral dose, all body weights were normal for both sexes. There were no abnormal physical signs noted during the observation period. Necropsy results were normal.

Based on this information it is considered that also ditin trisulfide reveals no acute oral toxicity up to the limit dose.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Quality of whole database:

Two GLP and OECD guideline compliant studies (423, 425) on different tin sulfides are available.

Acute toxicity: via inhalation route

Link to relevant study records

Reference

Endpoint:

acute toxicity: inhalation

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Read-Across Justification_SnS and SnS2 to Sn2S3

Reason / purpose for cross-reference:

read-across source

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 5.57 mg/L air

Based on:

other: calculated for ditin trisulfide

Exp. duration:

4 h

Mortality:

No mortality was observed.

Clinical signs:

other: Slight laboured respiration for some exposed animals on day 1 of exposure. No abnormaliies were detected during later observation period.

Body weight:

Normal body weight for all animals during the observation period was messured, with exeption of some animals during the first three days, who lost slightly body weight.

Gross pathology:

The exposure was associated with test item-related brown discolarion of lungs and enlarged lung-associated lymph nodes.

Conclusions:

The acute inhalation median lethal concentration (4hr LC50) for read-across substance Tin sulfide was considered to be greater than 5.03 mg/L.
Calculated for ditin trisulfide the 4hr LC50 is > 5.57 mg/L.

Executive summary:

The study was performed in accordance to test guidelines OPPTS 870.1300 and OECD guideline 403. The acute toxicity of read-across substance Tin sulfide after a 4 hour exposure at target concentration of 5 mg/L to 5 male and 5 female rats (CRL: (WI) Wistar strain) was determined. Rats were exposed using a nose-only exposure system followed by a 14 day observation period. Aerosol concentrations were measured gravimetrically. Particle size distribution was determined regularly during exposure period. Clinical observations and body weights were recorded and animals were subjected to a gross examination post mortem. All clinical observations (e.g. wet fur, fur staining) were considered to be related to exposure procedures and not as biologically significant. Normal body weights were noted during observation period with exception of slight body weight loss of some animals during the first three days of observation. In addition the exposure was associated with test item-related brown discoloration of lungs and enlarged lung-associated lymph nodes. But the mean lethal concentration after 4 hours is greater than 5.03 mg/L, because no death occurred.

Based on these results the 4 hr LC50 value calculated for ditin trisulfide is > 5.57 mg/L.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Quality of whole database:

Two GLP and OECD guideline compliant studies (403) on different tin sulfides are available.

Acute toxicity: via dermal route

Link to relevant study records

Reference

Endpoint:

acute toxicity: dermal

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

See attached Read-Across Justification_SnS and SnS2 to Sn2S3

Reason / purpose for cross-reference:

read-across source

Preliminary study:

All animals survived the 2000 mg/kg dermal application.

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 213 mg/kg bw

Based on:

other: calculated for ditin trisulfide

Mortality:

No mortality: All animals survived the 2000 mg/kg dermal application. Skin reactions were normal in all animals at all observations periods. Test article staining was noted on all dose sites at all observation periods. Body weight changes were normal. There were no abnormal physical and clinical signs noted during the observation period. Necropsy results were without any findings.

Clinical signs:

other: All animals survived the 2000 mg/kg dermal application. Skin reactions were normal in all animals at all observations periods. Test article staining was noted on all dose sites at all observation periods. Body weight changes were normal. There were no abn

Gross pathology:

All animals survived the 2000 mg/kg dermal application. Skin reactions were normal in all animals at all observations periods. Test article staining was noted on all dose sites at all observation periods. Body weight changes were normal. There were no abnormal physical and clinical signs noted during the observation period. Necropsy results were without any findings.

Other findings:

No other findings

Interpretation of results:

not classified

Remarks:

Criteria used for interpretation of results: EU

Conclusions:

In an acute toxicity study according to OECD guideline 402 the dermal LD50 of read-across substance tin sulfide was higher than 2000 mg/kg bw.
Calculated for ditin trisulfide the LD50 is > 2213 mg/kg bw .

Executive summary:

The study according to OECD guideline 402 was used to determine the potential for toxicity of read-across substance tin sulfide when applied dermally. All animals survived the 2000 mg/kg dermal application. Skin reactions were normal in all animals at all observations periods. Test item staining was noted on all dose sites at all observation periods. Body weight changes were normal. There were no abnormal physical and clinical signs noted during the observation period. Necropsy results were without any findings.

Based on this information it is considered that also ditin trisulfide reveals no acute dermal toxicity.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Quality of whole database:

Two GLP and OECD guideline compliant studies (402) on different tin sulfides are available.

Additional information

Oral:

The Acute Toxic Class Determination according to OECD guideline 423 revealed an LD50 is > 2000 mg/kg bw for tin sulfide in rats. Calculated for ditin trisulfide the LD50 value is > 2213 mg/kg bw.

An acute oral toxicity study on tin disulfide confirmed that tin sulfides in general are not acute oral toxic.

 

Inhalation:

The acute inhalation median lethal concentration (4hr LC50) for Tin sulfide was considered to be greater than 5.03 mg/L.

Calculated for ditin trisulfide the 4hr LC50 is > 5.57 mg/L.

An acute oral toxicity study on tin disulfide confirmed that tin sulfides in general are not acute toxic by inhalation.

 

Dermal:

In an acute toxicity study according to OECD guideline 402 the dermal LD50 of tin sulfide was higher than 2000 mg/kg bw.

Calculated for ditin trisulfide the LD50 is > 2213 mg/kg bw.

An acute oral toxicity study on tin disulfide confirmed that tin sulfides in general are not acute dermal toxic.

 

Justification for selection of acute toxicity – oral endpoint

Key GLP and guideline compliant study on a structural analogous read-across substance

 

Justification for selection of acute toxicity – inhalation endpoint

Key GLP and guideline compliant study on a structural analogous read-across substance

 

Justification for selection of acute toxicity – dermal endpoint

Key GLP and guideline compliant study on a structural analogous read-across substance

Justification for classification or non-classification

No classification and labelling is needed for acute toxicity (oral, inhalation, dermal) of ditin trisulfide according to Regulation No. 1272/2008 (CLP).