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EC number: 940-742-0
CAS number: -
Prescribed criteria of the test
1.Oxygen depletion in the
inoculum blank should not exceed 1.5 mg O2.L-1 after
2.The residual concentration
of oxygen in the test should not fall below 0.5 mg.L-
1 in any time.
3.The difference of extremes
of replicate values of the removal of test substance at the end of the
test should be
less than 20 %.
4.The percentage degradation
of the reference substance should reach the level for ready
biodegradability (min. 60 %) by 14 days.
5. If in a toxicity
test, containing both the test substance and the reference chemical,
less than 25 % degradation should occur in 14 days, the test substance
could be assumed to be inhibitory. The test series should be repeated,
if possible, using a lower concentration of test substance or a higher
concentration of inoculum.
The following values were attained
in the current study:
1.Oxygen depletion in the
inoculum blank reached 0.27 mg.L-1 on 28th day of the test.
2.The lowest concentration
of dissolved oxygen in bottles was 5.92 mg.L-1.
difference of two determinations of test substance degradation at the
end of test was 0.8 %.
4. The percentage
degradation of the reference substance has
reached the level for the ready biodegradability (min.60%)
by the 3rd day of the test.
5.In the toxicity test,
after 14 days of incubation the degradation of test and reference
substance was 33 %. Therefore the test substance is not inhibiting for
the used inoculum.
All the validity criteria
The test substance was
tested for the ready biological degradability in Closed Bottle Test.
The test was performed
Method C.4E - Closed Bottle Test,
Directive 92/69/EEC. Published in OJ L 383A, 1992.
The results of biological
degradation are related to calculated COD values of the test and
reference substance at the beginning of the test.
The test substance had
sufficient solubility in used mineral medium. The dosage from the stock
solution was carried out.
Sodium benzoate was used as
the reference substance.
COD of the test substance in
medium at the beginning of the main test: 3.42 mg.L-1
COD of reference substance
in medium at the beginning of the main test: 3.30 mg.L-1
In parallel to the main test
the toxicity test was performed.
As the chemical composition of
test substance is not exactly known the oxidized nitrogen forms
for the incidental correction of nitrification were determined .
test was performed at temperature of 20±1ºC
with the pH values of solutions 7-8 at the beginning of the
Validity of test
The prescribed validity
criteria in the test were fulfilled.
test substance was not inhibiting for the used inoculum.
all criteria of acceptability were met, this study is considered to be
study of ready biological degradability
the degradation of 2 %
of the test substance, Humic
acids, potassium salts, was
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