D-Valine, 3-mercapto-, lead complex

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline-Studie, ausführlich dokumentiert

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

D-Penicillamin-Bleisalz

IUPAC Name:

D-Penicillamin-Bleisalz

Constituent 2

Reference substance name:

Bleisalz der 2-Amino-3-mercapto-3-methylbuttersäure

IUPAC Name:

Bleisalz der 2-Amino-3-mercapto-3-methylbuttersäure

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Analytical purity: >99.0%
- Lot/batch No.: 120595
- Storage condition of test material: keine besonderen Maßnahmen
- Charakterisierung: graugelbes Pulver, charakteristischer Geruch
- Summenformel: C5H9NO2PbS

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0, 32 und 58 mg/L (nominal), nach 0 und 48 Stunden
- Sample storage conditions before analysis: tiefgefroren

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 50 mg Substanz und 50 mg PEG 600 wurden mit künstlichem Süßwasser auf 500 mL aufgefüllt und durch mehrstündiges Rühren gelöst.
- Controls: Hilfsstoffkontrolle 50 mg PEG 600 / 50 mL künstliches Süßwasser; 1 Kontrolle ohne Tiere für Bestimmung der PC-Daten
- Chemical name of vehicle: PEG 600

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Klon 5
- Source: Umweltbundesamt, Institut für Wasser-, Boden- und Lufthygiene, Berlin. In eigener Laborzucht weitergeführt.
- Age at study initiation: 6-24 Stunden
- Feeding during test: keine

ACCLIMATION
- Acclimation conditions: Temperatur 21,0-22,2°C (innerhalb 5 Tage vor Expositionsbeginn), Beleuchtung 12:12 Stunden, M4 Medium
- Type and amount of food: Algen
- Feeding frequency: an 6 Tagen/Woche

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

19,3 - 19,5 °C (0 Stunden)
19,7 - 20,4 °C (48 Stunden)

pH:

8,2 - 8,3 (0 Stunden)
8,0 - 8,4 (48 Stunden)

Dissolved oxygen:

8,8 - 9,0 mg/L (0 Stunden)
8,8 - 8,9 mg/L (48 Stunden)

Nominal and measured concentrations:

85 - 95% (Mittel 91%) der Nominalkonzentrationen wurden gemessen. Die Prüfsubstanz war über den Expositionszeitraum stabil.

Details on test conditions:

TEST SYSTEM
- Fill volume: 20 mL
- Aeration: keine
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 5
- No. of vessels per vehicle control (replicates): 5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: künstliches Süßwasser
- Ca/Mg: 2,04mmol/L Ca, 0,42 mmol/L Mg

TEST CONCENTRATIONS
- Test concentrations: 0, 10, 18, 32, 58, 95 mg/L (nominal)

Reference substance (positive control):

yes

Remarks:

Kaliumdichromat

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

32 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

65 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 95 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

32 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 5

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

61 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 6

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

95 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality of control: keine

Results with reference substance (positive control):

- EC50/LC50: 1,9 mg/L

Any other information on results incl. tables

Ergebnisse nach 24 und 48 Stunden:

Konzentration (mg/L)	Mortalität (%) nach 24 Stunden	Mortalität (%) nach 48 Stunden
0	0	0
0 (Hilfsstoff)	0	0
10	0	0
18	0	0
32	0	0
58	30	35
95	95	100

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Die 48h-EC50 für Daphnien wurde nach der EU-Methode C.2 getestet und beträgt 61 mg/L.