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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DIN 38412, part L11
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-hexyloxyethanol
EC Number:
203-951-1
EC Name:
2-hexyloxyethanol
Cas Number:
112-25-4
Molecular formula:
C8H18O2
IUPAC Name:
2-(hexyloxy)ethanol
Details on test material:
- Name of test material (as cited in study report): N-hexylglykol
- Purity: no data given

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution was prepared (1000 mg/L which was diluted in order to receive the required concentrations (geometrical series, factor 1.8)

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: Dr. U. Noack-Laboratorium, Hildesheim, Germany
- Method of breeding: 1 individuum per vial (filled with 70 ml)
- Feeding during test: no data


ACCLIMATION
- Acclimation period: no data

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
21 °C
pH:
7.52 - 7.69
Dissolved oxygen:
8.3 - 8.6 mg/l
Nominal and measured concentrations:
Nominal concentrations: 0, 32, 58, 100, 180, 320 and 580 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: beaker
- Type: open
- Material, size, fill volume: glass, 50ml, 20 ml
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to DIN 38412 part II, 8.2

OTHER TEST CONDITIONS
- Light intensity: diffuse, 10 (micro E/qm x s)

EFFECT PARAMETERS MEASURED: mobility after 3, 6, 24 and 48 h

Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
58 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
320 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
145 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
EC0= 1.0 mg/l
EC50= 1.45 mg/L
EC100 3.2 mg/L

Any other information on results incl. tables

Immobile individuals (%) after x hours (cumulative results)

 Nominal concentration (mg/L)  3  6  24  48
 0  0  0  0  0
 32  0  0  0  0
58  0  0  0  0
 100  0  0  0  0
 180  35  45  70  70
 320  100  100  100  100
 580  100  100  100  100

Applicant's summary and conclusion