Ethyl formate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Justification for type of information:

Data is from Experimental reports

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

To assess the extent of ocular damage caused by the test chemical to rabbit eyes

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl formate

EC Number:

203-721-0

EC Name:

Ethyl formate

Cas Number:

109-94-4

Molecular formula:

C3H6O2

IUPAC Name:

ethyl formate

Test material form:

liquid

Details on test material:

- Name of test material (IUPAC Name): Ethyl formate
- Molecular formula: C3H6O2
- Molecular weight: 74.0784 g/mol
- Smiles notation: O(CC)C=O
- InChI: 1S/C3H6O2/c1-2-5-3-4/h3H,2H2,1H3
- Substance type: Organic
- Physical state: Liquid

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Test system

Vehicle:

unchanged (no vehicle)

Controls:

yes, concurrent negative control

Amount / concentration applied:

undiluted approx 50 mg

Duration of treatment / exposure:

single exposure

Observation period (in vivo):

1, 24 hours till 8 days

Duration of post- treatment incubation (in vitro):

no data available

Number of animals or in vitro replicates:

no data available

Details on study design:

Sign explanation for skin and mucous membrane irritation:
R - redness; oe= Edema; Sch = Scaling ; N = necrosis; Tr = Cloudiness; Na = scar; (+) = questionable; + = easy; ++ = strong; +++ = very strong; * = unimpressive

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 8 days

Reversibility:

not specified

Remarks on result:

positive indication of irritation

Irritant / corrosive response data:

Ethyl formate causes marked irritation to the eyes

Any other information on results incl. tables

Eye Irritation (rabbits)

Application form: undiluted (1 * 50 mm3 or 50mg)	Findings after 1 hour	Findings after 24 hours	Findings after 8 days
	R + / * ++ / secretion	R + / o	*
Comparison : NaCl	*	*	*

 

Sign explanation for skin and mucous membrane irritation:

R - redness; oe= Edema; Sch = Scaling ; N = necrosis; Tr = Cloudiness; Na = scar; (+) = questionable; + = easy; ++ = strong; +++ = very strong; * = unimpressive

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritating to eyes) based on GHS criteria

Conclusions:

The test chemical causes marked irritation to the eyes as compared to the eyes treated with Sodium chloride.
Hence, the test chemical was considered to be irritating to rabbit eyes.

Executive summary:

A study was conducted to determine the extent of ocular damage caused by the test chemical to rabbit eyes.

Undiluted approx 50 mg of the test chemical was instilled into the eyes of rabbits and observed for effects. Sodium chloride was used for comparison of ocular responses. The ocular responses were observed and scored at 1, 24hours till 8 days after application.

The test chemical causes marked irritation to the eyes as compared to the eyes treated with  Sodium chloride.

Hence, the test chemical was considered to be irritating to rabbit eyes.